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Annexin A1 Monoclonal antibody

Annexin A1 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat







Cat no : 66344-1-Ig


Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35

Tested Applications

Positive WB detected inA549 cells, HeLa cells, A431 cells, NIH/3T3 cells, 4T1 cells, HCT 116 cells, Jurkat cells, K-562 cells, HepG2 cells
Positive IHC detected inhuman stomach tissue, mouse liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66344-1-Ig targets Annexin A1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin A1 fusion protein Ag17273
Full Name annexin A1
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001275
Gene Symbol ANXA1
Gene ID (NCBI) 301
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.


Product Specific Protocols
WB protocol for Annexin A1 antibody 66344-1-IgDownload protocol
IHC protocol for Annexin A1 antibody 66344-1-IgDownload protocol
IF protocol for Annexin A1 antibody 66344-1-IgDownload protocol
FC protocol for Annexin A1 antibody 66344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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