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CoraLite®594-conjugated Annexin A1 Monoclonal antibody

Annexin A1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E1B7

Cat no : CL594-66344

Synonyms

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated Annexin A1 Monoclonal anti (CL594-66344)

    IF Staining of A549 using CL594-66344

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 Annexin A1 antibody (CL594-66344, Clone: 1E1B7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of K-562 cells using CoraLite®594-conjugated Annexin A1 Monoclonal anti (CL594-66344)

    FC experiment of K-562 using CL594-66344

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Annexin A1 (CL594-66344, Clone:1E1B7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated Annexin A1 Monoclonal anti (CL594-66344)

    FC experiment of Jurkat using CL594-66344

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Annexin A1 (CL594-66344, Clone:1E1B7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA549 cells
Positive FC detected inK-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66344 targets Annexin A1 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Annexin A1 fusion protein Ag17273
Full Name annexin A1
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC001275
Gene symbol ANXA1
Gene ID (NCBI) 301
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Protocols

Product Specific Protocols
IF protocol for CL594 Annexin A1 antibody CL594-66344Download protocol
FC protocol for CL594 Annexin A1 antibody CL594-66344Download protocol
Standard Protocols
Click here to view our Standard Protocols