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WB analysis using 15416-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15416-1-AP (ANXA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 15416-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

ANXA9 Polyclonal antibody

Name: ANXA9 Polyclonal antibody
Brand: Proteintech
SKU: 15416-1-AP

ANXA9 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15416-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Annexin 31, Annexin 9, annexin A9, Annexin XXXI, ANX31, ANXA9, Pemphaxin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, MCF-7 cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below

Product Information

15416-1-AP targets ANXA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7702 Product name: Recombinant human ANXA9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC005830 Sequence: STMSVTGGKMAPSLTQEILSHLGLASKTAAWGTLGTLRTFLNFSVDKDAQRLLRAITGQGVDRSAIVDVLTNRSREQRQLISRNFQERTQQDLMKSLQAALSGNLERIVMALLQPTAQFDAQELRTALKASDSAVDVAIEILATRTPPQLQECLAVYKHNFQVEAVDDITSETSGILQDLLLALAKGGRDSYSGIIDYNLAEQDVQALQRAEGPSREETWVPVFTQRNPEHLIRVFDQYQRSTGQELEEAVQNRFHGDAQVALLGLASVIKNTPLYFADKLHQALQETEPNYQVLIRILISRCETDLLSIRAEFRKKFGKSLYSSLQDAVKGDCQSALLALCRAEDM Predict reactive species
Full Name	annexin A9
Calculated Molecular Weight	38 kDa
Observed Molecular Weight	38 kDa
GenBank Accession Number	BC005830
Gene Symbol	ANXA9
Gene ID (NCBI)	8416
RRID	AB_2057905
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O76027
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ANXA9 antibody 15416-1-AP	Download protocol
WB protocol for ANXA9 antibody 15416-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biol Reprod Rictor stability mediated by USP9X regulates embryo implantation by participating in lipid metabolism of endometrium Authors - Mingyu Peng View Article
human	WB,IHC	Biomedicines Targeting the ZMYM2-ANXA9 Axis with FLT3 Inhibitor G749 Overcomes Oxaliplatin Resistance in Colorectal Cancer Authors - Dezheng Lin View Article