AP2B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AP2B1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15690-1-AP

Synonyms

ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 15690-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15690-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 15690-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15690-1-AP

  IP Result of anti-AP2B1 (IP:15690-1-AP, 4ug; Detection:15690-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IF Staining of HeLa using 15690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15690-1-AP (AP2B1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, C6 cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Product Information

15690-1-AP targets AP2B1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP2B1 fusion protein Ag8248
• Full Name: adaptor-related protein complex 2, beta 1 subunit
• Calculated molecular weight: 105 kDa
• Observed molecular weight: 100 kDa-115 kDa
• GenBank accession number: BC006201
• Gene symbol: AP2B1
• Gene ID (NCBI): 163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.

Publications

Species	Application	Title
pig	WB,IF	Sci China Life Sci; NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.; Authors - Guoli Li
human	WB	J Virol; The G protein-coupled receptor FFAR2 promotes internalization during influenza A virus entry.; Authors - Guangwen Wang ; KD Validated
pig	WB	Viruses; Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection.; Authors - Yi Wang
human	WB	J Neuropathol Exp Neurol; Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene.; Authors - Yuriko Katsumata
human	IF	Biochem Biophys Res Commun; Trans-Golgi protein p230/golgin-245 is involved in phagophore formation.; Authors - Miwa Sohda
human	WB	J Exp Clin Cancer Res; GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway.; Authors - Xiao-Xin Zhang

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