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- KD/KO Validated
AP2B1 Polyclonal antibody
AP2B1 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, ELISA
Conjugate
Unconjugated
Cat no : 15690-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells, C6 cells, K-562 cells |
Positive IP detected in | K-562 cells |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
IF | See 2 publications below |
Product Information
15690-1-AP targets AP2B1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AP2B1 fusion protein Ag8248 |
Full Name | adaptor-related protein complex 2, beta 1 subunit |
Calculated molecular weight | 105 kDa |
Observed molecular weight | 100 kDa-115 kDa |
GenBank accession number | BC006201 |
Gene symbol | AP2B1 |
Gene ID (NCBI) | 163 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.
Protocols
Product Specific Protocols | |
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WB protocol for AP2B1 antibody 15690-1-AP | Download protocol |
IF protocol for AP2B1 antibody 15690-1-AP | Download protocol |
IP protocol for AP2B1 antibody 15690-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci China Life Sci NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry. | ||
J Virol The G protein-coupled receptor FFAR2 promotes internalization during influenza A virus entry.
| ||
Viruses Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection. | ||
J Neuropathol Exp Neurol Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene. | ||
Biochem Biophys Res Commun Trans-Golgi protein p230/golgin-245 is involved in phagophore formation. | ||
J Exp Clin Cancer Res GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway. |