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  • KD/KO Validated

AP2B1 Polyclonal antibody

AP2B1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 15690-1-AP


ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, K-562 cells, C6 cells, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15690-1-AP targets AP2B1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2B1 fusion protein Ag8248
Full Name adaptor-related protein complex 2, beta 1 subunit
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC006201
Gene Symbol AP2B1
Gene ID (NCBI) 163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.


Product Specific Protocols
WB protocol for AP2B1 antibody 15690-1-APDownload protocol
IF protocol for AP2B1 antibody 15690-1-APDownload protocol
IP protocol for AP2B1 antibody 15690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Emerg Microbes Infect

CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis

Authors - Xin Chen


Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein

Authors - Congyan Zhang

Sci China Life Sci

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.

Authors - Guoli Li

J Virol

The G protein-coupled receptor FFAR2 promotes internalization during influenza A virus entry.

Authors - Guangwen Wang
  • KD Validated


Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection.

Authors - Yi Wang

J Neuropathol Exp Neurol

Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene.

Authors - Yuriko Katsumata