AARE Polyclonal antibody

AARE Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14758-1-AP

Synonyms

AARE, ACPH, Acyl peptide hydrolase, Acylaminoacyl peptidase, APEH, APH, D3F15S2, D3S48E, DNF15S2, OPH, Oxidized protein hydrolase



Tested Applications

Positive WB detected inmouse liver tissue, COLO 320 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14758-1-AP targets AARE in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AARE fusion protein Ag6511
Full Name N-acylaminoacyl-peptide hydrolase
Calculated molecular weight 81 kDa
Observed molecular weight 66-80 kDa
GenBank accession numberBC000362
Gene symbol APEH
Gene ID (NCBI) 327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH.

Protocols

Product Specific Protocols
WB protocol for AARE antibody 14758-1-APDownload protocol
IHC protocol for AARE antibody 14758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

mSphere

Internalization of Erythrocyte Acylpeptide Hydrolase Is Required for Asexual Replication of Plasmodium falciparum.

Authors - Rubayet Elahi