Validation Data Gallery
|Positive WB detected in||human blood tissue, human brain tissue, human ileum tissue, mouse lung tissue, PC-13 cells|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human liver cancer tissue, human liver tissue, human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse liver tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14427-1-AP targets Apolipoprotein AI in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Apolipoprotein AI fusion protein Ag5793|
|Full Name||apolipoprotein A-I|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||26-30 kDa|
|GenBank accession number||BC005380|
|Gene ID (NCBI)||335|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
|Product Specific Protocols|
|WB protocol for Apolipoprotein AI antibody 14427-1-AP||Download protocol|
|IHC protocol for Apolipoprotein AI antibody 14427-1-AP||Download protocol|
|IF protocol for Apolipoprotein AI antibody 14427-1-AP||Download protocol|
|IP protocol for Apolipoprotein AI antibody 14427-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Alexandre (Verified Customer) (02-28-2019)
Detection of both human and mouse APOA1 protein by westernblotImmunoprecipitation of both human and mouse APOA1