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Apolipoprotein AI Polyclonal antibody
Apolipoprotein AI Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 14427-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human blood tissue, human brain tissue, human plasma, human ileum tissue |
Positive IHC detected in | human liver cancer tissue, human liver tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse liver tissue |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 29 publications below |
IHC | See 10 publications below |
IF | See 4 publications below |
IP | See 2 publications below |
Product Information
14427-1-AP targets Apolipoprotein AI in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Apolipoprotein AI fusion protein Ag5793 |
Full Name | apolipoprotein A-I |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 26-30 kDa |
GenBank Accession Number | BC005380 |
Gene Symbol | APOA1 |
Gene ID (NCBI) | 335 |
RRID | AB_2056524 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Apolipoprotein AI antibody 14427-1-AP | Download protocol |
IHC protocol for Apolipoprotein AI antibody 14427-1-AP | Download protocol |
IF protocol for Apolipoprotein AI antibody 14427-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein. | ||
Neuron Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. | ||
J Control Release Lipid-mediated protein corona regulation with increased apolipoprotein A-I recruitment for glioma targeting | ||
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. | ||
Theranostics Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment | ||
Br J Pharmacol A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alexandre (Verified Customer) (02-28-2019) | Detection of both human and mouse APOA1 protein by westernblotImmunoprecipitation of both human and mouse APOA1
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