Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14427-1-AP

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1



Tested Applications

Positive WB detected inhuman blood tissue, human brain tissue, human ileum tissue, mouse lung tissue, PC-13 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse liver tissue
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14427-1-AP targets Apolipoprotein AI in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag5793
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IHC protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IF protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IP protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Cell Res

Proteomic analysis of mouse ovaries during the prepubertal stages.

Authors - Jiaqiang Xiong
humanIHC

Br J Cancer

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Authors - Xiaohui Liu
humanWB

Aging (Albany NY)

Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients.

Authors - Wenjing Wang
humanWB

Biochem Biophys Rep

miR-33a-5p in small extracellular vesicles as non-invasive biomarker for oxaliplatin sensitivity in human colorectal cancer cells.

Authors - Shota Tanaka
mouseWB

J Sport Health Sci

Exercise promotes angiogenesis by enhancing endothelial cell fatty acid use via liver-derived extracellular vesicle miR-122-5p.

Authors - Jing Lou
mouseWB

Exp Ther Med

Effects of sepsis on the metabolism of sphingomyelin and cholesterol in mice with liver dysfunction.

Authors - Jiaqi Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alexandre (Verified Customer) (02-28-2019)

Detection of both human and mouse APOA1 protein by westernblotImmunoprecipitation of both human and mouse APOA1

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: WB 1/1000
  • Cell Tissue Type: HepG2 / Mouse Liver
Apolipoprotein AI Antibody Western Blot,Immunoprecipitation, validation (WB 1/1000 dilution) in HepG2 / Mouse Liver (Cat no:14427-1-AP)