|Positive WB detected in||A375 cells, PC-3 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C6 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
60100-1-Ig targets APOBEC3G in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||APOBEC3G fusion protein Ag7770|
|Full Name||apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC024268|
|Gene ID (NCBI)||60489|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.
Int J Clin Exp Med
APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.
Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.