APOE Polyclonal antibody

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• KD/KO Validated

APOE Polyclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 18254-1-AP

Synonyms

AD2, Apo E, APOE, apoe4, apolipoprotein E, LPG

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Validation Data Gallery

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  WB analysis using 18254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18254-1-AP (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18254-1-AP

  WB result of APOE antibody (18254-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOE transfected HepG2 cells.

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  IF Staining of HepG2 using 18254-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (18254-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 18254-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APOE (18254-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HuH-7 cells, HepG2 cells, human plasma, mouse serum
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

18254-1-AP targets APOE in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOE fusion protein Ag13070
• Full Name: apolipoprotein E
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-38 kDa
• GenBank Accession Number: BC003557
• Gene Symbol: APOE
• Gene ID (NCBI): 348
• RRID: AB_2878525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight. APOE can be detected 43kDa and 69kDa dimers (PMID: 9831633).

Publications

Species	Application	Title
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu
mouse	WB	J Genet Genomics; Comprehensive lipidomics in apoM-/- mice reveals an overall state of metabolic distress and attenuated hepatic lipid secretion into the circulation.; Authors - Yuanping Shi
human	WB	J Virol; SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.; Authors - Da-Yuan Chen
mouse	WB	Hum Cell; Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis.; Authors - Hao Meng
mouse	WB	Pulm Pharmacol Ther; Protective effects of adipose-derived biogenic nanoparticles on the pulmonary microvascular endothelial barrier in mice with ventilator-induced lung injury via the TRPV4/ROCK1 signalling pathway.; Authors - Qian Yu
mouse	WB	Autoimmunity; Inclisiran inhibits oxidized low-density lipoprotein-induced foam cell formation in Raw264.7 macrophages via activating the PPARγ pathway.; Authors - Zhaoping Wang