APOE Polyclonal antibody

APOE Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 18254-1-AP

Synonyms

AD2, Apo E, APOE, apoe4, apolipoprotein E, LPG



Tested Applications

Positive WB detected inHuH-7 cells, human plasma, mouse serum
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18254-1-AP is APOE Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOE fusion protein Ag13070
Full Name apolipoprotein E
Calculated molecular weight 36 kDa
Observed molecular weight 34-38 kDa
GenBank accession numberBC003557
Gene symbol APOE
Gene ID (NCBI) 348
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

Protocols

Product Specific Protocols
WB protocol for APOE antibody 18254-1-APDownload protocol
FC protocol for APOE antibody 18254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Br J Cancer

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Authors - Xiaohui Liu
humanWB

bioRxiv

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

Authors - Da-Yuan Chen
mouseWB

Autoimmunity

Inclisiran inhibits oxidized low-density lipoprotein-induced foam cell formation in Raw264.7 macrophages via activating the PPARγ pathway.

Authors - Zhaoping Wang
mouseWB

Pulm Pharmacol Ther

Protective effects of adipose-derived biogenic nanoparticles on the pulmonary microvascular endothelial barrier in mice with ventilator-induced lung injury via the TRPV4/ROCK1 signalling pathway.

Authors - Qian Yu
mouseWB

Hum Cell

Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis.

Authors - Hao Meng
humanWB

J Virol

SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.

Authors - Da-Yuan Chen