APOE Polyclonal antibody
APOE Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 18254-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HuH-7 cells, human plasma, mouse serum |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 11 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
Product Information
18254-1-AP targets APOE in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | APOE fusion protein Ag13070 |
Full Name | apolipoprotein E |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 34-38 kDa |
GenBank accession number | BC003557 |
Gene symbol | APOE |
Gene ID (NCBI) | 348 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight. APOE can be detected 43kDa and 69kDa dimers (PMID: 9831633).
Protocols
Product Specific Protocols | |
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WB protocol for APOE antibody 18254-1-AP | Download protocol |
FC protocol for APOE antibody 18254-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Br J Cancer A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline. | ||
J Genet Genomics Comprehensive lipidomics in apoM-/- mice reveals an overall state of metabolic distress and attenuated hepatic lipid secretion into the circulation. | ||
Hum Cell Serine protease HtrA2/Omi regulates adaptive mitochondrial reprogramming in the brain cortex after ischemia/reperfusion injury via UCP2-SIRT3-PGC1 axis. | ||
Pulm Pharmacol Ther Protective effects of adipose-derived biogenic nanoparticles on the pulmonary microvascular endothelial barrier in mice with ventilator-induced lung injury via the TRPV4/ROCK1 signalling pathway. | ||
Autoimmunity Inclisiran inhibits oxidized low-density lipoprotein-induced foam cell formation in Raw264.7 macrophages via activating the PPARγ pathway. |