Apolipoprotein H Monoclonal antibody
Apolipoprotein H Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
4D9A4
Cat no : 66074-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig heart tissue, human plasma, human heart tissue, pig plasma, human testis tissue, rat heart, mouse brain, rat brain |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human liver cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:800 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
The immunogen of 66074-1-Ig is Apolipoprotein H Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Apolipoprotein H fusion protein Ag17915 |
Full Name | apolipoprotein H (beta-2-glycoprotein I) |
Calculated molecular weight | 345 aa, 38 kDa |
Observed molecular weight | 50-53 kDa |
GenBank accession number | BC026283 |
Gene symbol | APOH |
Gene ID (NCBI) | 350 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.
Protocols
Product Specific Protocols | |
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WB protocol for Apolipoprotein H antibody 66074-1-Ig | Download protocol |
IHC protocol for Apolipoprotein H antibody 66074-1-Ig | Download protocol |
IF protocol for Apolipoprotein H antibody 66074-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Thromb Haemost Identification of APOH polymorphisms as common genetic risk factors for venous thrombosis in the Chinese population. | ||
Curr Med Sci Thrombophilia Caused by Beta2-Glycoprotein I Deficiency: In Vitro Study of a Rare Mutation in APOH Gene. |