Apolipoprotein H Monoclonal antibody

Apolipoprotein H Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4D9A4

Cat no : 66074-1-Ig

Synonyms

Anticardiolipin cofactor, APC inhibitor, Apo H, APOH, Apolipoprotein H, B2G1, B2GPI, Beta 2 glycoprotein 1, Beta 2 glycoprotein I, Beta(2)GPI, BG

---

Validation Data Gallery

• 

  WB analysis using 66074-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 66074-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66074-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig plasma using 66074-1-Ig

  pig plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66074-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human liver cancer using 66074-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: pig heart tissue, human plasma, human heart tissue, pig plasma, human testis tissue, rat heart, mouse brain, rat brain
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:100-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 66074-1-Ig is Apolipoprotein H Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Apolipoprotein H fusion protein Ag17915
• Full Name: apolipoprotein H (beta-2-glycoprotein I)
• Calculated molecular weight: 345 aa, 38 kDa
• Observed molecular weight: 50-53 kDa
• GenBank accession number: BC026283
• Gene symbol: APOH
• Gene ID (NCBI): 350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

Publications

Species	Application	Title
human	WB	J Thromb Haemost; Identification of APOH polymorphisms as common genetic risk factors for venous thrombosis in the Chinese population.; Authors - Liang Tang
human	WB	Curr Med Sci; Thrombophilia Caused by Beta2-Glycoprotein I Deficiency: In Vitro Study of a Rare Mutation in APOH Gene.; Authors - Xiao-Ping Zhang