Apolipoprotein H Monoclonal antibody

Apolipoprotein H Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4D9A4

Cat no : 66074-1-Ig

Synonyms

Anticardiolipin cofactor, APC inhibitor, Apo H, APOH, Apolipoprotein H, B2G1, B2GPI, Beta 2 glycoprotein 1, Beta 2 glycoprotein I, Beta(2)GPI, BG



Tested Applications

Positive WB detected inpig heart tissue, human plasma, human heart tissue, pig plasma, human testis tissue, rat heart, mouse brain, rat brain
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:100-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 66074-1-Ig is Apolipoprotein H Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Apolipoprotein H fusion protein Ag17915
Full Name apolipoprotein H (beta-2-glycoprotein I)
Calculated molecular weight 345 aa, 38 kDa
Observed molecular weight 50-53 kDa
GenBank accession numberBC026283
Gene symbol APOH
Gene ID (NCBI) 350
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in bloodcoagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein H antibody 66074-1-IgDownload protocol
IHC protocol for Apolipoprotein H antibody 66074-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Med Sci

Thrombophilia Caused by Beta2-Glycoprotein I Deficiency: In Vitro Study of a Rare Mutation in APOH Gene.

Authors - Xiao-Ping Zhang
humanWB

J Thromb Haemost

Identification of APOH polymorphisms as common genetic risk factors for venous thrombosis in the Chinese population.

Authors - Liang Tang