Validation Data Gallery
|Positive WB detected in||pig heart tissue, human plasma, human heart tissue, pig plasma, human testis tissue, rat heart, mouse brain, rat brain|
|Positive IHC detected in||human liver cancer tissue, human colon cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66074-1-Ig is Apolipoprotein H Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Apolipoprotein H fusion protein Ag17915|
|Full Name||apolipoprotein H (beta-2-glycoprotein I)|
|Calculated molecular weight||345 aa, 38 kDa|
|Observed molecular weight||50-53 kDa|
|GenBank accession number||BC026283|
|Gene ID (NCBI)||350|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.
|Product Specific Protocols|
|WB protocol for Apolipoprotein H antibody 66074-1-Ig||Download protocol|
|IHC protocol for Apolipoprotein H antibody 66074-1-Ig||Download protocol|
|IF protocol for Apolipoprotein H antibody 66074-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Thromb Haemost
Identification of APOH polymorphisms as common genetic risk factors for venous thrombosis in the Chinese population.
Curr Med Sci
Thrombophilia Caused by Beta2-Glycoprotein I Deficiency: In Vitro Study of a Rare Mutation in APOH Gene.