AQP1 Polyclonal antibody

AQP1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

64

Cat no : 20333-1-AP

Synonyms

Aquaporin 1, Aquaporin-CHIP, Aquaporin-1, Aquaporin1, AQP-1

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Validation Data Gallery

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  WB analysis using 20333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20333-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 20333-1-AP

  IP result of anti-AQP1 (IP:20333-1-AP, 4ug; Detection:20333-1-AP 1:40000) with mouse kidney tissue lysate 2160 ug.

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  IP experiment of mouse skeletal muscle using 20333-1-AP

  IP result of anti-AQP1 (IP:20333-1-AP, 4ug; Detection:20333-1-AP 1:1500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human breast cancer using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, human heart tissue, rat skeletal muscle, mouse kidney, rat kidney
• Positive IP detected in: mouse kidney tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse kidney tissue, human breast cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:3000-1:12000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 29 publications below
• IHC: See 23 publications below
• IF: See 23 publications below
• IP: See 2 publications below
• FC: See 2 publications below

Product Information

20333-1-AP targets AQP1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, bovine, goat, horse, cat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AQP1 fusion protein Ag14093
• Full Name: aquaporin 1 (Colton blood group)
• Calculated Molecular Weight: 269 aa, 29 kDa
• Observed Molecular Weight: 25-28 kDa, 35-50 kDa
• GenBank Accession Number: BC022486
• Gene Symbol: AQP1
• Gene ID (NCBI): 358
• RRID: AB_10666159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 35-50 kDa. (PMID:20965731,16508653, 1530176).

Publications

Species	Application	Title
pig	IHC	Kidney Int; Physiologic Homeostasis after Pig-to-Human Kidney Xenotransplantation; Authors - Eric Judd
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	IF	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
human	IHC	Proc Natl Acad Sci U S A; Direct visualization of the arterial wall water permeability barrier using CARS microscopy.; Authors - Bertrand M Lucotte
human	IHC,IF	Cancer Res; Stromal-Based Signatures for the Classification of Gastric Cancer.; Authors - Mark T Uhlik
		Cell Rep; ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells; Authors - Danping Lu

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