AQP1 Polyclonal antibody
AQP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 20333-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, rat skeletal muscle |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 12 publications below |
| IHC | See 16 publications below |
| IF | See 16 publications below |
| IP | See 1 publications below |
| FC | See 2 publications below |
Product Information
The immunogen of 20333-1-AP is AQP1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine, canine, Cat, Goat, Horse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AQP1 fusion protein Ag14093 |
| Full Name | aquaporin 1 (Colton blood group) |
| Calculated molecular weight | 269 aa, 29 kDa |
| Observed molecular weight | 25-28 kDa, 40-45 kDa |
| GenBank accession number | BC022486 |
| Gene symbol | AQP1 |
| Gene ID (NCBI) | 358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AQP1 antibody 20333-1-AP | Download protocol |
| IHC protocol for AQP1 antibody 20333-1-AP | Download protocol |
| IF protocol for AQP1 antibody 20333-1-AP | Download protocol |
| IP protocol for AQP1 antibody 20333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Methods Mol Biol Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells. | ||
FEBS Open Bio Escin suppresses HMGB1-induced overexpression of aquaporin-1 and increased permeability in endothelial cells. | ||
J Neurosci Methods A novel histochemical method of simultaneous detection by a single- or double-immunofluorescence and Bielschowsky's silver staining in teased rat sciatic nerves. | ||
Cell Death Dis AQP1 modulates tendon stem/progenitor cells senescence during tendon aging. | ||
IBRO Neurosci Rep Differences in the localization of AQP1 and expression patterns of AQP isoforms in rat and mouse sciatic nerve and changes in rat AQPs expression after nerve crush injury. |
