AQP1 Polyclonal antibody

AQP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20333-1-AP

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human heart tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20333-1-AP is AQP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity Cat, Cow, Dog, Goat, Horse, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AQP1 fusion protein Ag14093
Full Name aquaporin 1 (Colton blood group)
Calculated molecular weight 269 aa, 29 kDa
Observed molecular weight 25-28 kDa, 40-45 kDa
GenBank accession numberBC022486
Gene symbol AQP1
Gene ID (NCBI) 358
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

Protocols

Product Specific Protocols
WB protocol for AQP1 antibody 20333-1-APDownload protocol
IHC protocol for AQP1 antibody 20333-1-APDownload protocol
IP protocol for AQP1 antibody 20333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Methods Mol Biol

Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells.

Authors - Krithika Hariharan
humanWB,IF

FEBS Open Bio

Escin suppresses HMGB1-induced overexpression of aquaporin-1 and increased permeability in endothelial cells.

Authors - Changjun Chen

J Nutr Biochem

CSRP3 mediates polyphenols-induced cardioprotection in hypertension.

Authors - Carole Oudot
ratIHC,IF

J Neurosci Methods

A novel histochemical method of simultaneous detection by a single- or double-immunofluorescence and Bielschowsky's silver staining in teased rat sciatic nerves.

Authors - Edith Segura-Anaya
ratWB,IF

Cell Death Dis

AQP1 modulates tendon stem/progenitor cells senescence during tendon aging.

Authors - Minhao Chen
ratIHC

Exp Ther Med

Traditional Chinese medicine, Qing Ying Tang, ameliorates the severity of acute lung injury induced by severe acute pancreatitis in rats via the upregulation of aquaporin-1.

Authors - Zhenming Gao