AQP1 Polyclonal antibody

AQP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (6)





Cat no : 20333-1-AP


AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human heart tissue, rat skeletal muscle, mouse kidney, rat kidney
Positive IP detected inmouse kidney tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse kidney tissue, human breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, mouse kidney tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20333-1-AP targets AQP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Cat, Goat, Horse, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AQP1 fusion protein Ag14093
Full Name aquaporin 1 (Colton blood group)
Calculated Molecular Weight 269 aa, 29 kDa
Observed Molecular Weight 25-28 kDa, 35-50 kDa
GenBank Accession NumberBC022486
Gene Symbol AQP1
Gene ID (NCBI) 358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 35-50 kDa. (PMID:20965731,16508653, 1530176).


Product Specific Protocols
WB protocol for AQP1 antibody 20333-1-APDownload protocol
IHC protocol for AQP1 antibody 20333-1-APDownload protocol
IF protocol for AQP1 antibody 20333-1-APDownload protocol
IP protocol for AQP1 antibody 20333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Authors - Bojun Li

Proc Natl Acad Sci U S A

Direct visualization of the arterial wall water permeability barrier using CARS microscopy.

Authors - Bertrand M Lucotte

Cancer Res

Stromal-Based Signatures for the Classification of Gastric Cancer.

Authors - Mark T Uhlik

Clin Transl Med

NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.

Authors - Zhixin Liu

J Pathol

Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway.

Authors - Tomoki Kai

Cell Prolif

Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system.

Authors - Thao Thi Thanh Ngo