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AQP1 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 20333-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

AQP1 Antibody WB human skeletal muscle tissue 20333-1-AP

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20333-1-AP(AQP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

AQP1 Antibody IP mouse skeletal muscle tissue 20333-1-AP

IP Result of anti-AQP1 (IP:20333-1-AP, 4ug; Detection:20333-1-AP 1:1500) with mouse skeletal muscle tissue lysate 3600ug.

AQP1 Antibody IHC human kidney tissue 20333-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20333-1-AP( AQP1 Antibody) at dilution of 1:200 (under 10x lens)

AQP1 Antibody IHC human kidney tissue 20333-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20333-1-AP( AQP1 Antibody) at dilution of 1:200 (under 40x lens)

Species specificity:
human, mouse, rat, Cat, Cow, Dog, Goat, Horse, pig
Positive WB detected in:
human skeletal muscle tissue, human heart tissue, human placenta tissue, mouse pancreas tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected in:
mouse skeletal muscle tissue
Positive IHC detected in:
human kidney tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AQP1 fusion protein Ag14093
Full name:
aquaporin 1 (Colton blood group)
Calculated molecular weight:
269aa,29 kDa
Observed molecular weight:
25-28 kDa,40-45 kDa
GenBank accession number:
BC022486
Gene ID (NCBI):
358
Gene symbol
AQP1
Synonyms
AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

Background

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)


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