Validation Data Gallery
|Positive WB detected in||mouse skeletal muscle tissue, human heart tissue, rat skeletal muscle, mouse kidney, rat kidney|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human breast cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue, mouse kidney tissue|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20333-1-AP is AQP1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, canine, Cat, Goat, Horse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AQP1 fusion protein Ag14093|
|Full Name||aquaporin 1 (Colton blood group)|
|Calculated molecular weight||269 aa, 29 kDa|
|Observed molecular weight||25-28 kDa, 40-45 kDa|
|GenBank accession number||BC022486|
|Gene ID (NCBI)||358|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)
|Product Specific Protocols|
|WB protocol for AQP1 antibody 20333-1-AP||Download protocol|
|IHC protocol for AQP1 antibody 20333-1-AP||Download protocol|
|IF protocol for AQP1 antibody 20333-1-AP||Download protocol|
|IP protocol for AQP1 antibody 20333-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
Direct visualization of the arterial wall water permeability barrier using CARS microscopy.
Stromal-Based Signatures for the Classification of Gastric Cancer.
Clin Transl Med
NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.
Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway.
Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system.
Front Cell Dev Biol
Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition.