CoraLite® Plus 488-conjugated AQP1 Polyclonal antibody

AQP1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20333

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

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Validation Data Gallery

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  IF Staining of mouse kidney using CL488-20333

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite® Plus 488 AQP1 antibody (CL488-20333) at dilution of 1:200.

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  IF Staining of human breast cancer using CL488-20333

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 AQP1 antibody (CL488-20333) at dilution of 1:200.

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  IF Staining of human breast cancer using CL488-20333

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 AQP1 antibody (CL488-20333) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse kidney tissue, human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-20333 is AQP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AQP1 fusion protein Ag14093
• Full Name: aquaporin 1 (Colton blood group)
• Calculated Molecular Weight: 269 aa, 29 kDa
• Observed Molecular Weight: 25-28 kDa, 40-45 kDa
• GenBank Accession Number: BC022486
• Gene Symbol: AQP1
• Gene ID (NCBI): 358
• RRID: AB_2919170
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)