androgen receptor Monoclonal antibody

androgen receptor Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, Mouse, Rat and More (3)







Cat no : 66747-1-Ig


AIS, androgen receptor, AR, DHTR, Dihydrotestosterone receptor, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM

Tested Applications

Positive WB detected inLNCaP cells, human testis tissue, NCCIT cells
Positive IHC detected inhuman prostate cancer tissue, mouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman prostate cancer tissue, LNCaP cells

Recommended dilution

Western Blot (WB)WB : 1:600-1:3000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66747-1-Ig targets androgen receptor in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen androgen receptor fusion protein Ag17291
Full Name androgen receptor
Calculated Molecular Weight 914 aa, 99 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC132975
Gene Symbol AR
Gene ID (NCBI) 367
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AR, also named as DHTR and NR3C4, belongs to the nuclear hormone receptor family and NR3 subfamily. AR is a ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. AR is activated, but not phosphorylated, by HIPK3. Defects in AR are the cause of androgen insensitivity syndrome (AIS), previously known as testicular feminization syndrome (TFM), which is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Defects in AR are the cause of spinal and bulbar muscular atrophy X-linked type 1 (SMAX1) which also known as Kennedy disease. Defects in AR may play a role in metastatic prostate cancer. Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) which also known as Reifenstein syndrome. AR exists various isoforms with MW 110-120 kDa and 75-80 kDa. (PMID: 19244107 )


Product Specific Protocols
WB protocol for androgen receptor antibody 66747-1-IgDownload protocol
IHC protocol for androgen receptor antibody 66747-1-IgDownload protocol
IF protocol for androgen receptor antibody 66747-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Human prostate organoid generation and the identification of prostate development drivers using inductive rodent tissues

Authors - Parmveer Singh

Chin J Nat Med

Eucommia lignans alleviate the progression of diabetic nephropathy through mediating the AR/Nrf2/HO-1/AMPK axis in vivo and in vitro

Authors - Qi Huang


WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation

Authors - Kai Song

Gynecol Endocrinol

N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway

Authors - Yuan-Xue Jing


Effects of testosterone dose on depression-like behavior among castrated adult male rats

Authors - Zhongyu Ren