ARHGEF11 Recombinant antibody

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  IHC staining of human colon using 84149-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 84149-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84149-1-RR (ARHGEF11 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HuH-7 using 84149-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using ARHGEF11 antibody (84149-1-RR, Clone: 241427A4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84149-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84149-1-RR against Human ARHGEF11 were performed. The affinity constant is 1.09 nM.

ARHGEF11 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84149-1-RR

Clone No.241427A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

241427A4, GTRAP48, KIAA0380, PDZ RHOGEF

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HuH-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84149-1-RR targets ARHGEF11 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARHGEF11 fusion protein Ag33104
• Full Name: Rho guanine nucleotide exchange factor (GEF) 11
• Calculated Molecular Weight: 168 kDa
• Gene Symbol: ARHGEF11
• Gene ID (NCBI): 9826
• RRID: AB_3671713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15085
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF11 (Rho Guanine Nucleotide Exchange Factor 11), also known as PDZ-RhoGEF or GTRAP48. ARHGEF11 contains several functional domains, including a PDZ domain and a RhoGEF domain. The PDZ domain allows it to interact with various proteins, such as ZO-1 and plexins, while the RhoGEF domain enables it to activate Rho GTPases. ARHGEF11 is a multifunctional protein that plays crucial roles in cell adhesion, signal transduction, and development. Its dysregulation has been linked to several diseases, making it an important area of research for potential therapeutic applications.