ARPC1A Polyclonal antibody

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  WB analysis using 17538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17538-1-AP (ARPC1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ARPC1A Polyclonal Antibody for WB, ELISA

Cat No. 17538-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Arc40, ARPC1A, SOP2 like protein, SOP2Hs, SOP2L

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17538-1-AP targets ARPC1A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARPC1A fusion protein Ag11593
• Full Name: actin related protein 2/3 complex, subunit 1A, 41kDa
• Calculated Molecular Weight: 370 aa, 42 kDa
• Observed Molecular Weight: 37-42 kDa
• GenBank Accession Number: BC039594
• Gene Symbol: ARPC1A
• Gene ID (NCBI): 10552
• RRID: AB_3085527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92747
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARPC1A, also known as SOP2L, belongs to the WD repeat ARPC1 family. ARPC1A is most similar to ARPC1B, and the similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells (PMID: 9230079). ARPC1A functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (PMID: 8978670). Western blot analysis detected ARPC1A at an apparent molecular mass of 37-42 kDa.