Tested Applications
| Positive WB detected in | mouse brain tissue, 3T3-L1 cells, rat brain tissue |
| Positive IHC detected in | mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16127-1-AP targets CLMP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9111 Product name: Recombinant human ASAM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-229 aa of BC009371 Sequence: EIKRVAEEKVTLPCHHQLGLPEKDTLDIEWLLTDNEGNQKVVITYSSRHVYNNLTEEQKGRVAFASNFLAGDASLQIEPLKPSDEGRYTCKVKNSGRYVWSHVILKVLVRPSKPKCELEGELTEGSDLTLQCESSSGTEPIVYYWQRIREKEGEDERLPPKSRIDYNHPGRVLLQNLTMSYSGLYQCTAGNEAGKESCVVRVTVQYVQ Predict reactive species |
| Full Name | adipocyte-specific adhesion molecule |
| Calculated Molecular Weight | 373 aa, 41 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC009371 |
| Gene Symbol | CLMP |
| Gene ID (NCBI) | 79827 |
| RRID | AB_2878221 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6B4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLMP (Coxsackie- and adenovirus receptor-like membrane protein), also known as ACAM (adipocyte adhesion molecule) or ASAM, is a member of the CTX (cortical thymocyte marker in Xenopus) family of proteins. CTX family proteins are type I transmembrane proteins within the Ig superfamily that localize to junctional complexes between endothelial and epithelial cells and may play a role in cell-cell adhesion. CLMP is predominantly expressed in epithelial cells within different tissues and in the white adipose tissue. It may be involved in the cell-cell adhesion and may play a role in adipocyte differentiation and development of obesity.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CLMP antibody 16127-1-AP | Download protocol |
| WB protocol for CLMP antibody 16127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CAR (Coxsackievirus and Adenovirus Receptor) antibody, catalog number 16127-1-AP, has 1 total citation. It appears in Cell Microbiology (2021), used in Western blot on human samples. The cited study investigates how Porphyromonas gingivalis degrades CAR to facilitate lipopolysaccharide and peptidoglycan penetration through gingival epithelium, placing the research in the fields of periodontal microbiology and host–pathogen interactions at mucosal barriers.
| Species | Application | Title |
|---|---|---|
Cell Microbiol Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of coxsackievirus and adenovirus receptor. |
