CLMP Polyclonal antibody

CLMP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16127-1-AP

Synonyms

ACAM, adipocyte adhesion molecule, ASAM, CLMP, CXADR-like membrane protein

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Validation Data Gallery

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  WB analysis using 16127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16127-1-AP (CLMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 16127-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 16127-1-AP (CLMP antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 16127-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 16127-1-AP (CLMP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 16127-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 16127-1-AP (CLMP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, 3T3-L1 cells, rat brain tissue
• Positive IHC detected in: mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16127-1-AP targets CLMP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLMP fusion protein Ag9111
• Full Name: adipocyte-specific adhesion molecule
• Calculated Molecular Weight: 373 aa, 41 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC009371
• Gene Symbol: ASAM
• Gene ID (NCBI): 79827
• RRID: AB_2878221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLMP (Coxsackie- and adenovirus receptor-like membrane protein), also known as ACAM (adipocyte adhesion molecule) or ASAM, is a member of the CTX (cortical thymocyte marker in Xenopus) family of proteins. CTX family proteins are type I transmembrane proteins within the Ig superfamily that localize to junctional complexes between endothelial and epithelial cells and may play a role in cell-cell adhesion. CLMP is predominantly expressed in epithelial cells within different tissues and in the white adipose tissue. It may be involved in the cell-cell adhesion and may play a role in adipocyte differentiation and development of obesity.

Publications

Species	Application	Title
human	WB	Cell Microbiol; Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of coxsackievirus and adenovirus receptor.; Authors - Hiroki Takeuchi

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