ASCC2 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86086-1-RR (same clone as 86086-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86086-1-RR (ASCC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86086-1-RR (same clone as 86086-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86086-1-RR (ASCC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86086-1-RR (same clone as 86086-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (86086-1-RR, Clone: 250566D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86086-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02784-1

  The mean ASCC2 concentration was determined to be 113.7 ng/mL in HEK-293T cell extract based on a 1.2 mg/mL extract load, 127.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 82.7 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02784-1

  Cytometric bead array standard curve of MP02784-1, ASCC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86086-1-PBS. Detection antibody: 86086-2-PBS. Standard: Ag2100. Range: 3.125-200 ng/mL.

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  Affinity and Kinetic Characterization of 86086-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86086-1-RR against Human ASCC2 were performed. The affinity constant is 26.5 pM.

ASCC2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86086-1-PBS

Clone No.250566D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ASC-1 complex subunit p100, ASC1P100, RQT3

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Product Information

86086-1-PBS targets ASCC2 as part of a matched antibody pair:

MP02784-1: 86086-1-PBS capture and 86086-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2100

  Product name: Recombinant human ASCC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 408-757 aa of BC025368

  Sequence: RRKATDAKDPSVIEEPNGEPNGVTVTAEAVSQASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILACLEYYHYDPEQVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHKGKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISRRPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKAEARRMAFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRANHNRRTMADRKRSKGMIPS

• Full Name: activating signal cointegrator 1 complex subunit 2
• Calculated Molecular Weight: 757 aa, 86 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC025368
• Gene Symbol: ASCC2
• Gene ID (NCBI): 84164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H1I8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).