ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1B7C11

Cat no : 66346-1-Ig

Synonyms

ARSA, ARSA I, ARSA1, Arsenical pump driving ATPase, Arsenite stimulated ATPase, ASNA1, ATPase ASNA1, hARSA I, hASNA I, TRC40



Tested Applications

Positive WB detected inrat brain tissue, fetal human brain tissue, HEK-293 cells, Neuro-2a cells, pig brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66346-1-Ig targets ASNA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASNA1 fusion protein Ag7936
Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Calculated molecular weight 38.7 kDa
Observed molecular weight 37-41 kDa
GenBank accession numberBC002651
Gene symbol ASNA1
Gene ID (NCBI) 439
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

Protocols

Product Specific Protocols
WB protocol for ASNA1 antibody 66346-1-IgDownload protocol
IHC protocol for ASNA1 antibody 66346-1-IgDownload protocol
IF protocol for ASNA1 antibody 66346-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols