ASNA1 Monoclonal antibody

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  WB analysis using 66346-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66346-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66346-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66346-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66346-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66346-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66346-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66346-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66346-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66346-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 66346-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HEK-293 using 66346-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ASNA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66346-1-Ig

Clone No.1B7C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, chicken

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

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Tested Applications

• Positive WB detected in: Neuro-2a cells, fetal human brain tissue, pig brain tissue, HEK-293 cells, HeLa cells, rat brain tissue, rabbit brain tissue, mouse brain tissue, chicken brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66346-1-Ig targets ASNA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7936

  Product name: Recombinant human ASNA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-348 aa of BC002651

  Sequence: MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ

• Full Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• Calculated Molecular Weight: 38.7 kDa
• Observed Molecular Weight: 37-41 kDa
• GenBank Accession Number: BC002651
• Gene Symbol: ASNA1
• Gene ID (NCBI): 439
• RRID: AB_2881726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43681
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.