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ATF6 Monoclonal antibody
ATF6 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human, mouse, rat and More (1)
WB, IHC, IF, FC, ELISA
Cat no : 66563-1-Ig
Validation Data Gallery
|Positive WB detected in||U2OS cells, HeLa cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, MCF-7 cells, Jurkat cells, K-562 cells|
|Positive IHC detected in||human cervical cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 15 publications below|
|IF||See 1 publications below|
66563-1-Ig targets ATF6 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ATF6 fusion protein Ag21456|
|Full Name||activating transcription factor 6|
|Calculated molecular weight||75 kDa|
|Observed molecular weight||90-100 kDa|
|GenBank accession number||BC014969|
|Gene ID (NCBI)||22926|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.
|Product Specific Protocols|
|WB protocol for ATF6 antibody 66563-1-Ig||Download protocol|
|IHC protocol for ATF6 antibody 66563-1-Ig||Download protocol|
|IF protocol for ATF6 antibody 66563-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
POST1/C12ORF49 regulates the SREBP pathway by promoting site-1 protease maturation.
J Cell Biol
Antigen-derived peptides engage the ER stress sensor IRE1α to curb dendritic cell cross-presentation.
The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma
Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers.
A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.
Ecotoxicol Environ Saf
Role of endoplasmic reticulum stress in cadmium-induced hepatocyte apoptosis and the protective effect of quercetin.