ATF6B Polyclonal antibody

ATF6B Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15794-1-AP

Synonyms

ATF6 beta, ATF6B, CREB RP, CREBL1, G13, Protein G13



Tested Applications

Positive WB detected inJurkat cells, human testis tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

15794-1-AP targets ATF6B in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF6B fusion protein Ag8502
Full Name activating transcription factor 6 beta
Calculated molecular weight 703 aa, 77 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC008394
Gene symbol ATF6B
Gene ID (NCBI) 1388
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The endoplasmic reticulum (ER)-transmembrane proteins, ATF6 alpha (ATF6) and ATF6 beta (ATF6B), are cleaved during the ER stress response (ERSR). The resulting N-terminal fragments of both ATF6 isoforms, which have conserved basic leucine-zipper and DNA binding domains but divergent transcriptional activation domains, translocate to the nucleus where they bind to ER stress-response elements (ERSE) in ERSR, such as Bip/GRP78. This antibody could recognize ATF6 beta (110kd) and its cleaved forms(60,80kd )(PMID: 11256944)

Protocols

Product Specific Protocols
WB protocol for ATF6B antibody 15794-1-APDownload protocol
IHC protocol for ATF6B antibody 15794-1-APDownload protocol
IF protocol for ATF6B antibody 15794-1-APDownload protocol
IP protocol for ATF6B antibody 15794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neuropathol Exp Neurol

Altered Oligodendrocyte Maturation and Myelin Maintenance: The Role of Antiretrovirals in HIV-Associated Neurocognitive Disorders.

Authors - Brigid K Jensen
humanWB

Molecules

Sinulariolide Induced Hepatocellular Carcinoma Apoptosis through Activation of Mitochondrial-Related Apoptotic and PERK/eIF2α/ATF4/CHOP Pathway.

Authors - Chen Yi-Jen YJ
ratWB

Neural Regen Res

Neuroprotective mechanism of L-cysteine after subarachnoid hemorrhage.

Authors - Ye Xiong
ratWB

Toxicol Lett

Methamphetamine mediates apoptosis of vascular smooth muscle cells via the chop-related endoplasmic reticulum stress pathway.

Authors - Xiaohui Tan
humanWB

Proteomics

Evaluation of the hepatotoxic risk caused by lead acetate via skin exposure using a proteomic approach.

Authors - Jia-You Fang
mouseWB

Front Pharmacol

Geniposide Alleviates Isoproterenol-Induced Cardiac Fibrosis Partially via SIRT1 Activation in vivo and in vitro.

Authors - Ning Li