ATF6B Polyclonal antibody

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  WB analysis of Jurkat using 15794-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15794-1-AP (ATF6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15794-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15794-1-AP (ATF6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15794-1-AP

  IP result of anti-ATF6B (IP:15794-1-AP, 4ug; Detection:15794-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human brain using 15794-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15794-1-AP (ATF6B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15794-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15794-1-AP (ATF6B antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15794-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATF6B antibody (15794-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

ATF6B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15794-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CREB RP, cAMP-responsive element-binding protein-like 1, cAMP-dependent transcription factor ATF-6 beta, cAMP response element-binding protein-related protein, ATF6-beta

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Tested Applications

• Positive WB detected in: Jurkat cells, human testis tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

15794-1-AP targets ATF6B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8502

  Product name: Recombinant human ATF6B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-318 aa of BC008394

  Sequence: MAELMLLSEIADPTRFFTDNLLSPEDWGLQNSTLYSGLDEVAEEQTQLFRCPEQDVPFDGSSLDVGMDVSPSEPPWELLPIFPDLQVKSEPSSPCSSSSLSSESSRLSTEPSSEALGVGEVLHVKTESLAPPLCLLGDDPTSSFETVQINVIPTSDDSSDVQTKIEPVSPCSSVNSEASLLSADSSSQAFIGEEVLEVKTESLSPSGCLLWDVPAPSLGAVQISMGPSLDGSSGKALPTRKPPLQPKPVVLTTVPMPSRAVSPSTTVLLQSLVQPPPGTEEGEKGRAWWLTPVIPALWEAEAGESPEVRSLRPAWPTW

• Full Name: activating transcription factor 6 beta
• Calculated Molecular Weight: 703 aa, 77 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC008394
• Gene Symbol: ATF6B
• Gene ID (NCBI): 1388
• RRID: AB_2058912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99941
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The endoplasmic reticulum (ER)-transmembrane proteins, ATF6 alpha (ATF6) and ATF6 beta (ATF6B), are cleaved during the ER stress response (ERSR). The resulting N-terminal fragments of both ATF6 isoforms, which have conserved basic leucine-zipper and DNA binding domains but divergent transcriptional activation domains, translocate to the nucleus where they bind to ER stress-response elements (ERSE) in ERSR, such as Bip/GRP78. This antibody could recognize ATF6 beta (110kd) and its cleaved forms(60,80kd )(PMID: 11256944)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen
human	WB	Int J Mol Sci; Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss.; Authors - Francesca Potenza
mouse,human	WB,IHC,IF	Front Cell Dev Biol; miR-1260b inhibits periodontal bone loss by targeting ATF6β mediated regulation of ER stress; Authors - Chikako Hayashi ; KD Validated
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
rat	WB	Front Cell Dev Biol; Mfn2 Regulates High Glucose-Induced MAMs Dysfunction and Apoptosis in Podocytes via PERK Pathway; Authors - Yun Cao
rat	WB	Arch Toxicol; Correction to: Protective effects of dioscin against alcohol‑induced liver injury.; Authors - Tingting Xu