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  • KD/KO Validated

ATF6B Polyclonal antibody

ATF6B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15794-1-AP

Synonyms

ATF6 beta, ATF6B, CREB RP, CREBL1, G13, Protein G13



Tested Applications

Positive WB detected inJurkat cells, human testis tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15794-1-AP targets ATF6B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF6B fusion protein Ag8502
Full Name activating transcription factor 6 beta
Calculated molecular weight 703 aa, 77 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC008394
Gene symbol ATF6B
Gene ID (NCBI) 1388
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The endoplasmic reticulum (ER)-transmembrane proteins, ATF6 alpha (ATF6) and ATF6 beta (ATF6B), are cleaved during the ER stress response (ERSR). The resulting N-terminal fragments of both ATF6 isoforms, which have conserved basic leucine-zipper and DNA binding domains but divergent transcriptional activation domains, translocate to the nucleus where they bind to ER stress-response elements (ERSE) in ERSR, such as Bip/GRP78. This antibody could recognize ATF6 beta (110kd) and its cleaved forms(60,80kd )(PMID: 11256944)

Protocols

Product Specific Protocols
WB protocol for ATF6B antibody 15794-1-APDownload protocol
IHC protocol for ATF6B antibody 15794-1-APDownload protocol
IF protocol for ATF6B antibody 15794-1-APDownload protocol
IP protocol for ATF6B antibody 15794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IHC,IF

Front Cell Dev Biol

miR-1260b inhibits periodontal bone loss by targeting ATF6β mediated regulation of ER stress

Authors - Chikako Hayashi
  • KD Validated
mouse, ratWB

Arch Toxicol

Protective effects of dioscin against alcohol-induced liver injury.

Authors - Xu Tingting T
ratWB

Front Cell Dev Biol

Mfn2 Regulates High Glucose-Induced MAMs Dysfunction and Apoptosis in Podocytes via PERK Pathway

Authors - Yun Cao
ratWB

Arch Toxicol

Correction to: Protective effects of dioscin against alcohol‑induced liver injury.

Authors - Tingting Xu
mouseWB

Biochim Biophys Acta Mol Basis Dis

Involvement of Kisspeptin in androgen-induced hypothalamic endoplasmic reticulum stress and its rescuing effect in PCOS rats.

Authors - Chun Yuan
mouseWB

Front Pharmacol

Geniposide Alleviates Isoproterenol-Induced Cardiac Fibrosis Partially via SIRT1 Activation in vivo and in vitro.

Authors - Ning Li