ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11264-1-AP

Synonyms

APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12



Tested Applications

Positive WB detected inPC-3 cells, COLO 320 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

11264-1-AP targets ATG12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG12 fusion protein Ag1791
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated molecular weight 15 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC012266
Gene symbol ATG12
Gene ID (NCBI) 9140
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

Protocols

Product Specific Protocols
WB protocol for ATG12 antibody 11264-1-APDownload protocol
IHC protocol for ATG12 antibody 11264-1-APDownload protocol
IF protocol for ATG12 antibody 11264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int J Biochem Cell Biol

Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy.

Authors - Chunyang Du
humanWB

Biochem J

Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.

Authors - Chunyang Du