ATG16L1 Polyclonal antibody

ATG16L1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 19812-1-AP


APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, IBD10, WDR30

Tested Applications

Positive WB detected inmouse spleen tissue, MCF-7 cells, Jurkat cells, HEK-293T cells
Positive IP detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19812-1-AP is ATG16L1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG16L1 fusion protein Ag13844
Full Name ATG16 autophagy related 16-like 1 (S. cerevisiae)
Calculated molecular weight 607 aa, 68 kDa
Observed molecular weight 63-71 kDa
GenBank accession numberBC000061
Gene symbol ATG16L1
Gene ID (NCBI) 55054
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)


Product Specific Protocols
WB protocol for ATG16L1 antibody 19812-1-APDownload protocol
IP protocol for ATG16L1 antibody 19812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Phase separation of Nur77 mediates celastrol-induced mitophagy by promoting the liquidity of p62/SQSTM1 condensates.

Authors - Shuang-Zhou Peng


AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.

Authors - Ashwinkumar Subramenium Ganapathy

Curr Biol

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Authors - Anna Ulbricht

Cancer Lett

Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy.

Authors - Bo You

Clin Transl Immunology

Circular RNA TRAPPC6B inhibits intracellular Mycobacterium tuberculosis growth while inducing autophagy in macrophages by targeting microRNA-874-3p.

Authors - Hou-Long Luo

Phytother Res

Modification of lysine deacetylation regulates curcumol-induced necroptosis through autophagy in hepatic stellate cells.

Authors - Sumin Sun


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 2000
  • Cell Tissue Type: Caco2, Mice colon

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 to 2000
  • Cell Tissue Type: Caco2, Mice colon

X (Verified Customer) (01-18-2021)

It is OK to use it in WB with human postmortem brain lysate. There is band with MW as expected, although there is other unidentified bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human postmortem brain lysate
ATG16L1 Antibody Western Blot validation (1:1000 dilution) in human postmortem brain lysate (Cat no:19812-1-AP)