ATG4D Recombinant monoclonal antibody

• 

  WB analysis of A549 using 83951-4-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 83951-4-RR (ATG4D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat liver using 83951-4-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83951-4-RR (ATG4D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83951-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83951-4-RR against Human ATG4D were performed. The affinity constant is below 1 pM.

ATG4D Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83951-4-RR

Clone No.241035G8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

AUT-like 4 cysteine endopeptidase, AUTL4, ATG, APG4D, APG4 D

---

Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83951-4-RR targets ATG4D in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10177

  Product name: Recombinant human ATG4D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 123-474 aa of BC068992

  Sequence: TYRRDFPPLPGGCLTSDCGWGCMLRSGQMMLAQGLLLHFLPRDWTWAEGMGLGPPELSGSASPSRYHGPARWMPPRWAQGAPELEQERRHRQIVSWFADHPRAPFGLHRLVELGQSSGKKAGDWYGPSLVAHILRKAVESCSDVTRLVVYVSQDCTVYKADVARLVARPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRCELCLGIMGGKPRHSLYFIGYQDDFLLYLDPHYCQPTVDVSQADFPLESFHCTSPRKMAFAKMDPSCTVGFYAGDRKEFETLCSELTRVLSSSSATERYPMFTLAEGHAQDHSLDDLCSQLAQPTLRLPRTGRLLRAKRPSSEDFVFL

• Full Name: ATG4 autophagy related 4 homolog D (S. cerevisiae)
• Calculated Molecular Weight: 474 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC068992
• Gene Symbol: ATG4D
• Gene ID (NCBI): 84971
• RRID: AB_3671530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86TL0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.