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  • KD/KO Validated

ATG7 Monoclonal antibody

ATG7 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B5A10

Cat no : 67341-1-Ig

Synonyms

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HepG2 cells, pig spleen tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67341-1-Ig targets ATG7 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATG7 fusion protein Ag29172
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated molecular weight 78 kDa
Observed molecular weight 68-78 kDa
GenBank accession numberBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protocols

Product Specific Protocols
WB protocol for ATG7 antibody 67341-1-IgDownload protocol
IHC protocol for ATG7 antibody 67341-1-IgDownload protocol
IF protocol for ATG7 antibody 67341-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function.

Authors - Yan Cao
  • KD Validated
ratWB

Biol Pharm Bull

PMID: 31787713

Authors - Akihiro Inamura
ratWB

Mol Med Rep

Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy.

Authors - Zhongzhong Liu
humanWB

Eur J Pharmacol

Latcripin-7A from Lentinula edodes C91-3 induces apoptosis, autophagy, and cell cycle arrest at G1 phase in human gastric cancer cells via inhibiting PI3K/Akt/mTOR signaling.

Authors - Syed Riaz Ud Din
mouseWB

Front Physiol

SHANK3 Co-ordinately Regulates Autophagy and Apoptosis in Myocardial Infarction.

Authors - Wanrong Man
humanWB

Am J Transl Res

Combination effect of curcumin with docetaxel on the PI3K/AKT/mTOR pathway to induce autophagy and apoptosis in esophageal squamous cell carcinoma.

Authors - Lian Deng