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ATG7 Monoclonal antibody

ATG7 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B5A10

Cat no : 67341-1-Ig

Synonyms

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7



Tested Applications

Positive WB detected inLNCaP cells, 4T1 cells, Jurkat cells, pig spleen tissue, HepG2 cells, HeLa cells, HEK-293 cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

67341-1-Ig targets ATG7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATG7 fusion protein Ag29172
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated molecular weight 78 kDa
Observed molecular weight 68-78 kDa
GenBank accession numberBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
RRIDAB_2882599
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protocols

Product Specific Protocols
WB protocol for ATG7 antibody 67341-1-IgDownload protocol
IHC protocol for ATG7 antibody 67341-1-IgDownload protocol
IF protocol for ATG7 antibody 67341-1-IgDownload protocol
FC protocol for ATG7 antibody 67341-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Cancers (Basel)

Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.

Authors - David P Dannheisig
mouseWB

J Cell Physiol

Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway

Authors - Jingru Guo
mouseWB

Stem Cell Res Ther

Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.

Authors - Jie Zhou
mouseWB

Phytomedicine

Trillin inhibits myoblast differentiation via increasing autophagy.

Authors - Weilong Dai
mouseWB

Front Pharmacol

Cardamonin Reduces Acetaminophen-Induced Acute Liver Injury in Mice via Activating Autophagy and NFE2L2 Signaling.

Authors - Qiushi Xu