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- KD/KO Validated
ATG7 Monoclonal antibody
ATG7 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 67341-1-Ig
Validation Data Gallery
|Positive WB detected in
|LNCaP cells, 4T1 cells, Jurkat cells, pig spleen tissue, HepG2 cells, HeLa cells, HEK-293 cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells
|Positive IHC detected in
|human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:500-1:2000
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.50 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
67341-1-Ig targets ATG7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
|Human, Mouse, Rat, Pig
|human, mouse, rat, canine
|Host / Isotype
|Mouse / IgG2a
|ATG7 fusion protein Ag29172
|ATG7 autophagy related 7 homolog (S. cerevisiae)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
|Product Specific Protocols
|WB protocol for ATG7 antibody 67341-1-Ig
|IHC protocol for ATG7 antibody 67341-1-Ig
|IF protocol for ATG7 antibody 67341-1-Ig
|FC protocol for ATG7 antibody 67341-1-Ig
|Click here to view our Standard Protocols
Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.
J Cell Physiol
Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway
Stem Cell Res Ther
Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.
Trillin inhibits myoblast differentiation via increasing autophagy.
Cardamonin Reduces Acetaminophen-Induced Acute Liver Injury in Mice via Activating Autophagy and NFE2L2 Signaling.