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- KD/KO Validated
GABARAPL1 Polyclonal antibody
GABARAPL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (2)
WB, IP, IHC, IF, FC, ELISA
Cat no : 11010-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, rat brain tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||mouse brain tissue, human ovary tumor tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Chloroquine treated HeLa cells, Chloroquine treated HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 6 publications below|
|WB||See 41 publications below|
|IHC||See 6 publications below|
|IF||See 18 publications below|
|IP||See 2 publications below|
11010-1-AP targets GABARAPL1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey, prawn|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GABARAPL1 fusion protein Ag1473|
|Full Name||GABA(A) receptor-associated protein like 1|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||16 kDa|
|GenBank accession number||BC009309|
|Gene ID (NCBI)||23710|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.
|Product Specific Protocols|
|WB protocol for GABARAPL1 antibody 11010-1-AP||Download protocol|
|IHC protocol for GABARAPL1 antibody 11010-1-AP||Download protocol|
|IF protocol for GABARAPL1 antibody 11010-1-AP||Download protocol|
|IP protocol for GABARAPL1 antibody 11010-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Markers of autophagy are downregulated in failing human heart after mechanical unloading.
J Extracell Vesicles
Secretion of pro-angiogenic extracellular vesicles during hypoxia is dependent on the autophagy-related protein GABARAPL1.
FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells.
J Clin Invest
Autophagy is essential for mouse sense of balance.
GABARAPL1 antibodies: target one protein, get one free!
The role of GABARAPL1/GEC1 in autophagic flux and mitochondrial quality control in MDA-MB-436 breast cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
H (Verified Customer) (04-06-2020)
I could not detect GABARAP L1 in HEK293T and HCT116 cells. It is possible that the expression level of GABARAP L1 in these two cell lines are extremely low. In proteomics analysis of the HEK293T whole cell lysates, however, we could detect GABARAP L1.