• Featured Product
  • KD/KO Validated

GABARAPL1-Specific Polyclonal antibody

GABARAPL1-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11010-1-AP

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, HepG2 cells, human heart tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue, human ovary tumor tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11010-1-AP targets GABARAPL1-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, prawns, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABARAPL1-Specific fusion protein Ag1473
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC009309
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.

Protocols

Product Specific Protocols
WB protocol for GABARAPL1-Specific antibody 11010-1-APDownload protocol
IHC protocol for GABARAPL1-Specific antibody 11010-1-APDownload protocol
IF protocol for GABARAPL1-Specific antibody 11010-1-APDownload protocol
IP protocol for GABARAPL1-Specific antibody 11010-1-APDownload protocol
FC protocol for GABARAPL1-Specific antibody 11010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Exp Med

Heterozygous Tbk1 loss has opposing effects in early and late stages of ALS in mice.

Authors - David Brenner
prawnsWB

Ecotoxicol Environ Saf

Evaluating expression of autophagy-related genes in oriental river prawn Macrobrachium nipponense as potential biomarkers for hypoxia exposure.

Authors - Shengming Sun
humanWB,IF

Autophagy

Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.

Authors - Alexander Agrotis
humanWB

Front Oncol

GABARAPL1 Promotes AR+ Prostate Cancer Growth by Increasing FL-AR/AR-V Transcription Activity and Nuclear Translocation.

Authors - Bing Su
  • KD Validated
humanWB,IHC

Cell Death Dis

Triggering a switch from basal- to luminal-like breast cancer subtype by the small-molecule diptoindonesin G via induction of GABARAPL1.

Authors - Minmin Fan
  • KD Validated
mouseWB

BMC Pulm Med

Pulmonary inflammation-induced alterations in key regulators of mitophagy and mitochondrial biogenesis in murine skeletal muscle.

Authors - Pieter A Leermakers

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (04-06-2020)

I could not detect GABARAP L1 in HEK293T and HCT116 cells. It is possible that the expression level of GABARAP L1 in these two cell lines are extremely low. In proteomics analysis of the HEK293T whole cell lysates, however, we could detect GABARAP L1.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T and HCT116
GABARAPL1-Specific Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:11010-1-AP)