GABARAPL1 Polyclonal antibody

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  WB analysis using 11010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11010-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11010-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11010-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11010-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11010-1-AP

  IP result of anti-GABARAPL1 (IP:11010-1-AP, 3ug; Detection:11010-1-AP 1:500) with mouse liver tissue lysate 5000ug.

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  IHC staining of mouse brain using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11010-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11010-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using GABARAPL1-Specific antibody (11010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 11010-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using GABARAPL1-Specific antibody (11010-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11010-1-AP

  Immunofluorescent analysis of Chloroquine treated HepG2 cells using 11010-1-AP (GABARAPL1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

GABARAPL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11010-1-AP

Publications(66)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, Early estrogen-regulated protein

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, rat brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse brain tissue, human ovary tumor tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Chloroquine treated HeLa cells, Chloroquine treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 52 publications below
• IHC: See 6 publications below
• IF: See 23 publications below
• IP: See 3 publications below

Product Information

11010-1-AP targets GABARAPL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, prawn
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GABARAPL1 fusion protein Ag1473
• Full Name: GABA(A) receptor-associated protein like 1
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC009309
• Gene Symbol: GABARAPL1
• Gene ID (NCBI): 23710
• RRID: AB_2294415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0R8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene, and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy, like GABARAP and GABARAPL2, and may be considered an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle, and at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.

Publications

Species	Application	Title
human	WB	Circulation; Markers of autophagy are downregulated in failing human heart after mechanical unloading.; Authors - Kassiotis Christos C
human	WB,IP,IF	J Extracell Vesicles; Secretion of pro-angiogenic extracellular vesicles during hypoxia is dependent on the autophagy-related protein GABARAPL1.; Authors - Tom G Keulers
mouse	WB	Cell Metab; FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells.; Authors - Zhao Jinghui J
mouse	WB	Autophagy; Analysis of ATG4C function in vivo; Authors - Isaac Tamargo-Gómez
mouse	WB,IF	J Clin Invest; Autophagy is essential for mouse sense of balance.; Authors - Mari?o Guillermo G
human	WB	Autophagy; GABARAPL1 antibodies: target one protein, get one free!; Authors - Le Grand Jaclyn Nicole JN

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Margaux (Verified Customer) (06-07-2024)

Efficient to immunoprecipitate GABARAPL1 but I used another antibody to see it because it didn't work for my western-blot in my experimental conditions.

• Applications: Immunoprecipitation

FH

H (Verified Customer) (04-06-2020)

I could not detect GABARAP L1 in HEK293T and HCT116 cells. It is possible that the expression level of GABARAP L1 in these two cell lines are extremely low. In proteomics analysis of the HEK293T whole cell lysates, however, we could detect GABARAP L1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T and HCT116

GABARAPL1 Antibody Western Blot validation (1:1000 dilution) in HEK293T and HCT116 (Cat no:11010-1-AP)