ATOH1 Polyclonal antibody

ATOH1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 21215-1-AP

Synonyms

ATH1, ATOH1, atonal homolog 1 (Drosophila), bHLHa14, HATH1, MATH 1, Protein atonal homolog 1



Tested Applications

Positive WB detected inmouse small intestine tissue, Neuro-2a cells, HepG2 cells
Positive IF detected inmouse brain tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21215-1-AP is ATOH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATOH1 fusion protein Ag15615
Full Name atonal homolog 1 (Drosophila)
Calculated molecular weight 354 aa, 38 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC069594
Gene symbol ATOH1
Gene ID (NCBI) 474
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.

Protocols

Product Specific Protocols
WB protocol for ATOH1 antibody 21215-1-APDownload protocol
IF protocol for ATOH1 antibody 21215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mousechIP

Dev Cell

Dual Regulatory Functions of SUFU and Targetome of GLI2 in SHH Subgroup Medulloblastoma.

Authors - Wen-Chi Yin
humanIF

Front Cell Dev Biol

Generation of Vestibular Tissue-Like Organoids From Human Pluripotent Stem Cells Using the Rotary Cell Culture System.

Authors - Cristiana Mattei
mouseIF

Cell Rep

Atoh1 Directs Regeneration and Functional Recovery of the Mature Mouse Vestibular System.

Authors - Zahra N Sayyid
mouseWB

Nat Metab

Intestinal MYC modulates obesity-related metabolic dysfunction.

Authors - Yuhong Luo
mouseIF

Neuroscience

Wls expression in the rhombic lip orchestrates the embryonic development of the mouse cerebellum.

Authors - Joanna Yeung
humanWB

Int J Mol Sci

SCF/c-KIT Signaling Increased Mucin2 Production by Maintaining Atoh1 Expression in Mucinous Colorectal Adenocarcinoma.

Authors - Ping Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Stephen (Verified Customer) (11-04-2020)

ATOH1 antibody was tested on embryonic chicken tissue. Staining showed nuclear localisation in inner ear vestibular crista in sox2 positive prosensory region of 6 day old embryo

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Embryonic chicken inner ear tissue
ATOH1 Antibody Immunofluorescence validation (1:300 dilution) in Embryonic chicken inner ear tissue (Cat no:21215-1-AP)
FH

Roland (Verified Customer) (09-17-2020)

We tested several ATOH1 antibodies and this is the only one which turned out to be specific as confirmed bei knockdown experiments.

  • Applications: Western Blot
  • Cell Tissue Type: human cancer cells
FH

Biren (Verified Customer) (08-31-2020)

I used human GBM stem cells as a negative control to validate the specificity of this antibody. The cells used were confirmed by RNAseq to not express ATOH1, yet all cells were positively stained at the previously used concentration of 1/500, indicating that this antibody is not specific.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: human glioblastoma stem cells
ATOH1 Antibody Immunofluorescence validation (1/500 dilution) in human glioblastoma stem cells (Cat no:21215-1-AP)
FH

Erithelgi (Verified Customer) (09-25-2019)

The antibody detects a band of the expected size, in cells that induce GFP-ATOH1 under a DOX promoter. No bands detected in non-induced cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: medulloblastoma
FH

Nicole (Verified Customer) (07-23-2019)

Used to stain cultured stem cells for differentiation markers.

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Cell Tissue Type: Stem Cell culture