|Positive WB detected in||Neuro-2a cells, mouse brain tissue, HepG2 cells, mouse small intestine tissue, rat brain tissue|
|Positive IF detected in||mouse brain tissue, HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21215-1-AP is ATOH1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATOH1 fusion protein Ag15615|
|Full Name||atonal homolog 1 (Drosophila)|
|Calculated molecular weight||354 aa, 38 kDa|
|Observed molecular weight||38-40 kDa|
|GenBank accession number||BC069594|
|Gene ID (NCBI)||474|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.
IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.
Nat Cell Biol
Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.
J Clin Invest
Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.
Proc Natl Acad Sci U S A
Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation
Addiction of Merkel cell carcinoma to MUC1-C identifies a potential new target for treatment.
Novel cell types and developmental lineages revealed by single-cell RNA-seq analysis of the mouse crista ampullaris.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Stephen (Verified Customer) (11-04-2020)
ATOH1 antibody was tested on embryonic chicken tissue. Staining showed nuclear localisation in inner ear vestibular crista in sox2 positive prosensory region of 6 day old embryo
Roland (Verified Customer) (09-17-2020)
We tested several ATOH1 antibodies and this is the only one which turned out to be specific as confirmed bei knockdown experiments.
Biren (Verified Customer) (08-31-2020)
I used human GBM stem cells as a negative control to validate the specificity of this antibody. The cells used were confirmed by RNAseq to not express ATOH1, yet all cells were positively stained at the previously used concentration of 1/500, indicating that this antibody is not specific.
Erithelgi (Verified Customer) (09-25-2019)
The antibody detects a band of the expected size, in cells that induce GFP-ATOH1 under a DOX promoter. No bands detected in non-induced cells.
Nicole (Verified Customer) (07-23-2019)
Used to stain cultured stem cells for differentiation markers.