ATOH1 Polyclonal antibody

ATOH1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 21215-1-AP


ATH1, ATOH1, atonal homolog 1 (Drosophila), bHLHa14, HATH1, MATH 1, Protein atonal homolog 1

Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue, HepG2 cells, mouse small intestine tissue, rat brain tissue
Positive IF detected inHepG2 cells, mouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21215-1-AP targets ATOH1 in WB, IF, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATOH1 fusion protein Ag15615
Full Name atonal homolog 1 (Drosophila)
Calculated Molecular Weight 354 aa, 38 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC069594
Gene Symbol ATOH1
Gene ID (NCBI) 474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.


Product Specific Protocols
WB protocol for ATOH1 antibody 21215-1-APDownload protocol
IF protocol for ATOH1 antibody 21215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

Decoding the spatiotemporal regulation of transcription factors during human spinal cord development

Authors - Yingchao Shi


IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.

Authors - Xun Lin

Nat Cell Biol

Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.

Authors - Donglim Esther Park

J Clin Invest

Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.

Authors - Patrick J Atkinson

Proc Natl Acad Sci U S A

Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation

Authors - Tianli Qin


Addiction of Merkel cell carcinoma to MUC1-C identifies a potential new target for treatment.

Authors - Yoshihiro Morimoto


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Yin (Verified Customer) (12-20-2021)

It did not work.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Cerebella granule cell

Stephen (Verified Customer) (11-04-2020)

ATOH1 antibody was tested on embryonic chicken tissue. Staining showed nuclear localisation in inner ear vestibular crista in sox2 positive prosensory region of 6 day old embryo

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Embryonic chicken inner ear tissue
ATOH1 Antibody Immunofluorescence validation (1:300 dilution) in Embryonic chicken inner ear tissue (Cat no:21215-1-AP)

Roland (Verified Customer) (09-17-2020)

We tested several ATOH1 antibodies and this is the only one which turned out to be specific as confirmed bei knockdown experiments.

  • Applications: Western Blot
  • Cell Tissue Type: human cancer cells

Biren (Verified Customer) (08-31-2020)

I used human GBM stem cells as a negative control to validate the specificity of this antibody. The cells used were confirmed by RNAseq to not express ATOH1, yet all cells were positively stained at the previously used concentration of 1/500, indicating that this antibody is not specific.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: human glioblastoma stem cells
ATOH1 Antibody Immunofluorescence validation (1/500 dilution) in human glioblastoma stem cells (Cat no:21215-1-AP)

Erithelgi (Verified Customer) (09-25-2019)

The antibody detects a band of the expected size, in cells that induce GFP-ATOH1 under a DOX promoter. No bands detected in non-induced cells.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: medulloblastoma

Nicole (Verified Customer) (07-23-2019)

Used to stain cultured stem cells for differentiation markers.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Cell Tissue Type: Stem Cell culture