ATP1A1 Monoclonal antibody, PBS Only (Detector)

ATP1A1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Cat No. 68735-2-PBS
Clone No.1E9B7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

1E9B7, ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

68735-2-PBS targets ATP1A1 as part of a matched antibody pair:

MP50088-1: 68735-1-PBS capture and 68735-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP1A1 fusion protein Ag5990 Predict reactive species
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05023
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

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