ATP1A2 Recombinant antibody, PBS Only

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  WB analysis using 84999-4-RR (same clone as 84999-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84999-4-RR (ATP1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84999-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 84999-4-RR (same clone as 84999-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84999-4-RR (ATP1A2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84999-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84999-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84999-4-RR against Human ATP1A2 were performed. The affinity constant is 4.12 nM.

ATP1A2 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84999-4-PBS

Clone No.242414G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Sodium pump subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, EC:7.2.2.13, ATP1A 2, alpha 2 ATPase

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Product Information

84999-4-PBS targets ATP1A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP1A2 fusion protein Ag10489
• Full Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Calculated Molecular Weight: 1020 aa, 112 kDa
• Observed Molecular Weight: 97-100 kDa
• GenBank Accession Number: BC052271
• Gene Symbol: ATP1A2
• Gene ID (NCBI): 477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50993
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4. The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. For optimal WB detection with ATP1A2, we do not recommend boiling the sample after lysis.