Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, various samples, Jurkat cells, mouse heart tissue, rat heart tissue, mouse brain tissue, rat brain tissue, mouse liver tissue, rat liver tissue, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse kidney tissue, human colon tissue, human kidney tissue, human liver tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14676-1-AP targets ATP5A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, zebrafish, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6385 Product name: Recombinant human ATP5A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 203-553 aa of BC064562 Sequence: GRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA Predict reactive species |
| Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC064562 |
| Gene Symbol | ATP5A1 |
| Gene ID (NCBI) | 498 |
| RRID | AB_2061761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25705 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo-containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ATP5A1 antibody 14676-1-AP | Download protocol |
| IF protocol for ATP5A1 antibody 14676-1-AP | Download protocol |
| IHC protocol for ATP5A1 antibody 14676-1-AP | Download protocol |
| IP protocol for ATP5A1 antibody 14676-1-AP | Download protocol |
| WB protocol for ATP5A1 antibody 14676-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP5A1 Polyclonal antibody (catalog #14676-1-AP) has 233 citations. These appear across high-impact journals including Science, Circulation, Cell Research, Cell Stem Cell, Autophagy, Nature Communications, Molecular Cell, Nature Chemical Biology, Cell Reports, EMBO Journal, PNAS, and FASEB Journal, among others. Research fields span mitochondrial biology, oxidative phosphorylation, mitophagy, cancer metabolism, cardiomyopathy, neurodegeneration, diabetes, ferroptosis, and inflammation. Applications are predominantly Western blot, with some IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Science Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists | ||
Circulation Nuclear Receptor NR1D1 Regulates Abdominal Aortic Aneurysm Development by Targeting the Mitochondrial Tricarboxylic Acid Cycle Enzyme Aconitase-2 | ||
Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies | ||
Cell Stem Cell Context-dependent roles of mitochondrial LONP1 in orchestrating the balance between airway progenitor versus progeny cells | ||
Bone Res Organelle-tuning condition robustly fabricates energetic mitochondria for cartilage regeneration | ||
Autophagy Hypoxia-induced BNIP3 facilitates the progression and metastasis of uveal melanoma by driving metabolic reprogramming |
Reviews
What customers say
All three reviews are 5-star, focused on Western Blot. Users report clear, specific bands at the expected ~55 kDa molecular weight across diverse samples including human cell lines (K562, KCL22), iPSC-derived neurons, and mouse heart tissue. Dilutions ranged from 1:1000 to 1:8000, and the antibody performed well with both ECL and fluorescent secondary detection. One user noted absence of a band in THP-1 cells, likely cell-line specific. Overall, reviewers describe it as a reliable, high-quality antibody for ATP5A1 detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Edward (Verified Customer) (11-15-2022) | Used with 12 ug protein lysate ATP5A1 works well, both with ECL detection and fluorescent secondary antibodies it gives a clear band for K562 and KCL22, though no band was apparent in THP-1 (this could be cell line specific).
|
XB (Verified Customer) (10-29-2020) | It probes ATP5A1 clearly with a right MW 55 kD.
 |
Siting (Verified Customer) (09-04-2020) | Good antibody
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