ATP5A1 Polyclonal antibody

ATP5A1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 14676-1-AP


ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM

Tested Applications

Positive WB detected inHeLa cells, various samples, HepG2 cells, Jurkat cells, mouse heart tissue, rat heart tissue, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human kidney tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14676-1-AP targets ATP5A1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5A1 fusion protein Ag6385
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC064562
Gene Symbol ATP5A1
Gene ID (NCBI) 498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo-containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.


Product Specific Protocols
WB protocol for ATP5A1 antibody 14676-1-APDownload protocol
IHC protocol for ATP5A1 antibody 14676-1-APDownload protocol
IF protocol for ATP5A1 antibody 14676-1-APDownload protocol
IP protocol for ATP5A1 antibody 14676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Authors - Liang Guo

Mol Cell

Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery

Authors - Jasmin Adriana Schafer

Nat Chem Biol

Small molecule agonist of mitochondrial fusion repairs mitochondrial dysfunction

Authors - Yingjie Guo

Sci Adv

Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.

Authors - Qiqi Guo

Nat Commun

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Authors - Zhisheng Xu

J Exp Med

FNIP1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics.

Authors - Yujing Yin


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate ATP5A1 works well, both with ECL detection and fluorescent secondary antibodies it gives a clear band for K562 and KCL22, though no band was apparent in THP-1 (this could be cell line specific).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: K562, KCL22 and THP-1

XB (Verified Customer) (10-29-2020)

It probes ATP5A1 clearly with a right MW 55 kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human IPSC-derived neurons
ATP5A1 Antibody Western Blot validation (1:2000 dilution) in human IPSC-derived neurons (Cat no:14676-1-AP)

Siting (Verified Customer) (09-04-2020)

Good antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart
ATP5A1 Antibody Western Blot validation (1:1000 dilution) in Mouse heart (Cat no:14676-1-AP)