ATP5F1 Polyclonal antibody
ATP5F1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IHC, IF, CoIP, ELISA
Cat no : 15999-1-AP
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, mouse skeletal muscle tissue, mouse liver tissue|
|Positive IHC detected in||human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human skin tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|WB||See 19 publications below|
|IHC||See 1 publications below|
|IF||See 1 publications below|
|CoIP||See 1 publications below|
15999-1-AP targets ATP5F1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP5F1 fusion protein Ag8571|
|Full Name||ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1|
|Calculated molecular weight||256 aa, 29 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC005366|
|Gene ID (NCBI)||515|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
|Product Specific Protocols|
|WB protocol for ATP5F1 antibody 15999-1-AP||Download protocol|
|IHC protocol for ATP5F1 antibody 15999-1-AP||Download protocol|
|IF protocol for ATP5F1 antibody 15999-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.
Part Fibre Toxicol
Transcriptomics of single dose and repeated carbon black and ozone inhalation co-exposure highlight progressive pulmonary mitochondrial dysfunction.
Free Radic Biol Med
Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3.
A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins.
Cell Death Dis
Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.
J Mol Biol
COA6 Facilitates Cytochrome c Oxidase Biogenesis as Thiol-reductase for Copper Metallochaperones in Mitochondria.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
siting (Verified Customer) (07-13-2020)
very good signal
Yan (Verified Customer) (01-28-2020)
It worked very well with mouse heart tissue for western blot.