ATP5F1 Polyclonal antibody
ATP5F1 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15999-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, mouse heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human skin tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 15 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15999-1-AP targets ATP5F1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP5F1 fusion protein Ag8571 |
| Full Name | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 |
| Calculated molecular weight | 256 aa, 29 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC005366 |
| Gene symbol | ATP5F1 |
| Gene ID (NCBI) | 515 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP5F1 antibody 15999-1-AP | Download protocol |
| IHC protocol for ATP5F1 antibody 15999-1-AP | Download protocol |
| IF protocol for ATP5F1 antibody 15999-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer. | ||
Biomed Pharmacother Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma. | ||
Virol J Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. | ||
J Agric Food Chem A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells. | ||
J Cell Biochem F1 FO ATP Synthase Is Expressed at the Surface of Embryonic Rat Heart-Derived H9c2 Cells and Is Affected by Cardiac-Like Differentiation. | ||
Genes (Basel) Heat Stress Impairs the Physiological Responses and Regulates Genes Coding for Extracellular Exosomal Proteins in Rat. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH siting (Verified Customer) (07-13-2020) | very good signal
 |
FH Yan (Verified Customer) (01-28-2020) | It worked very well with mouse heart tissue for western blot.
|
