ATP5H Recombinant antibody, PBS Only

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  WB analysis using 86174-2-RR (same clone as 86174-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86174-2-RR (ATP5H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86174-2-RR (same clone as 86174-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5H antibody (86174-2-RR, Clone: 250751G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86174-2-PBS in a different storage buffer formulation.

ATP5H Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86174-2-PBS

Clone No.250751G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ATP synthase peripheral stalk subunit d, ATP5, ATP5JD, ATP5PD, ATPQ

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Product Information

86174-2-PBS targets ATP5H in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP5H fusion protein Ag11429
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• Calculated Molecular Weight: 137 aa, 16 kDa
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC032245
• Gene Symbol: ATP5H
• Gene ID (NCBI): 10476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75947
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.