Validation Data Gallery
|Positive WB detected in||A549 cells, mouse placenta tissue, mouse liver tissue, mouse heart tissue, mouse colon tissue, Caco-2 cells, HCT116 cells, HEK-293 cells, HeLa cells, HepG2 cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue, human testis tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10994-1-AP targets ATP5O in WB, IHC, IF, FC applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP5O fusion protein Ag1458|
|Full Name||ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||23-25 kDa|
|GenBank accession number||BC021233|
|Gene ID (NCBI)||539|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.
|Product Specific Protocols|
|WB protocol for ATP5O antibody 10994-1-AP||Download protocol|
|IHC protocol for ATP5O antibody 10994-1-AP||Download protocol|
|IF protocol for ATP5O antibody 10994-1-AP||Download protocol|
|FC protocol for ATP5O antibody 10994-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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