ATR Polyclonal antibody

• Featured Product
• KD/KO Validated

ATR Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (3)

Cat no : 19787-1-AP

Synonyms

ATR, FRAP related protein 1, FRP1, MEC1, SCKL, SCKL1

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Validation Data Gallery

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  WB analysis using 19787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19787-1-AP (ATR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19787-1-AP

  WB result of ATR antibody (19787-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATR transfected HeLa cells.

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  IP experiment of mouse testis using 19787-1-AP

  IP result of anti-ATR(IP:19787-1-AP, 4ug; Detection:19787-1-AP 1:500) with mouse testis tissue lysate 2040 ug.

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  IHC staining of mouse testis using 19787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19787-1-AP (ATR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19787-1-AP (ATR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 20 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

19787-1-AP targets ATR in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ataxia telangiectasia and Rad3 related
• Calculated molecular weight: 301 kDa
• Observed molecular weight: 250-290 kDa
• GenBank accession number: NM_001184
• Gene symbol: ATR
• Gene ID (NCBI): 545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATR, also named as FRP1, belongs to the PI3/PI4-kinase family and ATM subfamily. ATR is a serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR recognizes the substrate consensus sequence [ST]-Q. ATR phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. It is required for FANCD2 ubiquitination. It is critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. ATR catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) which is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. The antibody can recognize all the isoforms of ATR.

Publications

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	IHC	ACS Nano; Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.; Authors - Yakun Wu
human	WB	Nat Commun; ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage.; Authors - Mingdong Liu
human	WB	Cancer Res; Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.; Authors - Zhe Sun
human	WB	Oncogene; Antifungal agent Terbinafine restrains tumor growth in preclinical models of hepatocellular carcinoma via AMPK-mTOR axis.; Authors - Er-Bin Zhang
human,mouse	WB	Am J Cancer Res; Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation.; Authors - Lu Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (01-17-2023)

I have used this antibody for human keratinocytes, cardiomyocytes, mouse skin and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin

FH

Marina (Verified Customer) (06-26-2022)

HCC1937 human breast cancer cells untreated (1) and treated with a PARP inhibitor (2). Primary antibody was incubated at 4ºC overnight in rotation. A single band >170 kDa could be observed in both conditions. Ponceau staining was used as total protein loading control.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human breast cancer cells

ATR Antibody Western Blot validation (1:500 dilution) in Human breast cancer cells (Cat no:19787-1-AP)

FH

WEI (Verified Customer) (03-08-2022)

Good target band with backgrouds

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart