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ATR Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 19787-1-AP

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Con: 42 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

ATR Antibody WB HeLa cells 19787-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 19787-1-AP(ATR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ATR Antibody IHC mouse testis tissue 19787-1-AP

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 19787-1-AP (ATR antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ATR Antibody IHC mouse testis tissue 19787-1-AP

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 19787-1-AP (ATR antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ATR Antibody IP mouse testis tissue 19787-1-AP

IP result of anti-ATR(IP:19787-1-AP, 4ug; Detection:19787-1-AP 1:300) with mouse testis tissue lysate 4000 ug

ATR Antibody IF HeLa cells 19787-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 19787-1-AP (ATR antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells
Positive IP detected in:
mouse testis tissue
Positive IHC detected in:
mouse testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:300-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
ataxia telangiectasia and Rad3 related
Calculated molecular weight:
301 kDa
Observed molecular weight:
250-290 kDa
GenBank accession number:
NM_001184
Gene ID (NCBI):
545
Gene symbol
ATR
Synonyms
ATR, FRAP related protein 1, FRP1, MEC1, SCKL, SCKL1

Background

ATR, also named as FRP1, belongs to the PI3/PI4-kinase family and ATM subfamily. ATR is a serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR recognizes the substrate consensus sequence [ST]-Q. ATR phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. It is required for FANCD2 ubiquitination. It is critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. ATR catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) which is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. The antibody can recognize all the isoforms of ATR.


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