ATR Polyclonal antibody

• Featured Product
• KD/KO Validated

ATR Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 19787-1-AP

Synonyms

ATR, FRAP related protein 1, FRP1, MEC1, SCKL, SCKL1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 19787-1-AP

  WB result of ATR antibody (19787-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATR transfected HeLa cells.

• 

  WB analysis of HeLa using 19787-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19787-1-AP (ATR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 19787-1-AP

  IP result of anti-ATR (IP:19787-1-AP, 4ug; Detection:19787-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

• 

  IHC staining of mouse testis using 19787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19787-1-AP (ATR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19787-1-AP (ATR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19787-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19787-1-AP (ATR antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below

Product Information

19787-1-AP targets ATR in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ataxia telangiectasia and Rad3 related
• Calculated molecular weight: 301 kDa
• Observed molecular weight: 250-290 kDa
• GenBank accession number: NM_001184
• Gene symbol: ATR
• Gene ID (NCBI): 545
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATR, also named as FRP1, belongs to the PI3/PI4-kinase family and ATM subfamily. ATR is a serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR recognizes the substrate consensus sequence [ST]-Q. ATR phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. It is required for FANCD2 ubiquitination. It is critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. ATR catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) which is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. The antibody can recognize all the isoforms of ATR.

Publications

Species	Application	Title
human	WB	Clin Genet; Methylmalonic Aciduria Cblb Type: Characterization Of Two Novel Mutations And Mitochondrial Dysfunction Studies.; Authors - S Brasil
human	WB	J Cell Mol Med; Potent USP10/13 antagonist spautin-1 suppresses melanoma growth via ROS-mediated DNA damage and exhibits synergy with cisplatin.; Authors - Jia Guo
human	WB	J Exp Clin Cancer Res; UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.; Authors - Jingyuan Sun
mouse	WB	Plant Foods Hum Nutr; Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.; Authors - Jie Wei
mouse	WB	J Agric Food Chem; Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.; Authors - Jie Wei
human	WB	Med Oncol; PMID: 31372848; Authors - Jingrui Jin