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ATR Polyclonal antibody

ATR Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19787-1-AP

Synonyms

ATR, FRAP related protein 1, FRP1, MEC1, SCKL, SCKL1



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19787-1-AP targets ATR in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ataxia telangiectasia and Rad3 related
Calculated molecular weight 301 kDa
Observed molecular weight 250-290 kDa
GenBank accession numberNM_001184
Gene symbol ATR
Gene ID (NCBI) 545
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATR, also named as FRP1, belongs to the PI3/PI4-kinase family and ATM subfamily. ATR is a serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR recognizes the substrate consensus sequence [ST]-Q. ATR phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. It is required for FANCD2 ubiquitination. It is critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. ATR catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) which is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. The antibody can recognize all the isoforms of ATR.

Protocols

Product Specific Protocols
WB protocol for ATR antibody 19787-1-APDownload protocol
IHC protocol for ATR antibody 19787-1-APDownload protocol
IP protocol for ATR antibody 19787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
mouseIHC

ACS Nano

Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.

Authors - Yakun Wu
humanWB

Nat Commun

ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage.

Authors - Mingdong Liu
humanWB

Cancer Res

Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.

Authors - Zhe Sun
humanWB

Oncogene

Antifungal agent Terbinafine restrains tumor growth in preclinical models of hepatocellular carcinoma via AMPK-mTOR axis.

Authors - Er-Bin Zhang
human,mouseWB

Am J Cancer Res

Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation.

Authors - Lu Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Priya (Verified Customer) (01-17-2023)

I have used this antibody for human keratinocytes, cardiomyocytes, mouse skin and liver tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, mouse Liver and skin
FH

Marina (Verified Customer) (06-26-2022)

HCC1937 human breast cancer cells untreated (1) and treated with a PARP inhibitor (2). Primary antibody was incubated at 4ºC overnight in rotation. A single band >170 kDa could be observed in both conditions. Ponceau staining was used as total protein loading control.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human breast cancer cells
ATR Antibody Western Blot validation (1:500 dilution) in Human breast cancer cells (Cat no:19787-1-AP)
FH

WEI (Verified Customer) (03-08-2022)

Good target band with backgrouds

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart