|Positive WB detected in||HeLa cells, mouse testis tissue|
|Positive IP detected in||mouse testis tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
19787-1-AP targets ATR in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||ataxia telangiectasia and Rad3 related|
|Calculated molecular weight||301 kDa|
|Observed molecular weight||250-290 kDa|
|GenBank accession number||NM_001184|
|Gene ID (NCBI)||545|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ATR, also named as FRP1, belongs to the PI3/PI4-kinase family and ATM subfamily. ATR is a serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR recognizes the substrate consensus sequence [ST]-Q. ATR phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. It is required for FANCD2 ubiquitination. It is critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. ATR catalyze the reaction: ATP + a protein = ADP + a phosphoprotein. Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) which is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance. The antibody can recognize all the isoforms of ATR.
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Graphene Oxide Causes Disordered Zonation Due to Differential Intralobular Localization in the Liver.
ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage.
Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.
Antifungal agent Terbinafine restrains tumor growth in preclinical models of hepatocellular carcinoma via AMPK-mTOR axis.
J Cell Mol Med
Potent USP10/13 antagonist spautin-1 suppresses melanoma growth via ROS-mediated DNA damage and exhibits synergy with cisplatin.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Priya (Verified Customer) (01-17-2023)
I have used this antibody for human keratinocytes, cardiomyocytes, mouse skin and liver tissues
Marina (Verified Customer) (06-26-2022)
HCC1937 human breast cancer cells untreated (1) and treated with a PARP inhibitor (2). Primary antibody was incubated at 4ºC overnight in rotation. A single band >170 kDa could be observed in both conditions. Ponceau staining was used as total protein loading control.
WEI (Verified Customer) (03-08-2022)
Good target band with backgrouds