Ataxin-3/ATXN3 Monoclonal antibody, PBS Only (Capture)

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  WB analysis of MCF-7 using 67057-1-Ig (same clone as 67057-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67057-1-Ig (same clone as 67057-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67057-1-Ig (same clone as 67057-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67057-1-Ig (same clone as 67057-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67057-1-Ig (same clone as 67057-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67057-1-Ig (same clone as 67057-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67057-1-Ig (same clone as 67057-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67057-1-Ig (same clone as 67057-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATX3,ATXN3 antibody (67057-1-Ig, Clone: 1F7E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67057-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51611-1

  Cytometric bead array sample test of MP51611-1, ATX3,ATXN3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67057-1-PBS. Detection antibody: 67057-2-PBS.

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  Cytometric bead array standard curve of MP51611-1

  Cytometric bead array standard curve of MP51611-1, ATX3,ATXN3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67057-1-PBS. Detection antibody: 67057-2-PBS. Standard:Ag6627. Range: 3.125-400 ng/mL.

Ataxin-3/ATXN3 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67057-1-PBS

Clone No.1F7E10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

ATX3,ATXN3, ATXN3, 1F7E10, AT3, ataxin 3

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Product Information

67057-1-PBS targets Ataxin-3/ATXN3 as part of a matched antibody pair:

MP51611-1: 67057-1-PBS capture and 67057-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6627

  Product name: Recombinant human ATX3,ATXN3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-370 aa of BC033711

  Sequence: MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKK

• Full Name: ataxin 3
• Calculated Molecular Weight: 370 aa, 43 kDa
• Observed Molecular Weight: 41-45 kDa
• GenBank Accession Number: BC033711
• Gene Symbol: ATXN3
• Gene ID (NCBI): 4287
• RRID: AB_2882368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54252
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.