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  • KD/KO Validated

Ataxin 2 Polyclonal antibody

Ataxin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21776-1-AP

Synonyms

ataxin 2, ATX2, ATXN2, SCA2, TNRC13



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, Neuro-2a cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21776-1-AP is Ataxin 2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, mosue
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ataxin 2 fusion protein Ag16470
Full Name ataxin 2
Calculated molecular weight 1313 aa, 140 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC114546
Gene symbol ATXN2
Gene ID (NCBI) 6311
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Background

 ATXN2 (Ataxin-2) is a eukaryotic RNA‐binding protein that is conserved across species. It contains two LSm domains (RNA binding), a PAM2 motif (association with poly(A)-binding protein), and an N-terminal polyglutamine tract (PMID: 25027299). Ataxin-2 plays crucial roles at different stages of the regulation of posttranslational gene expression. ATXN2 is also directly implicated in the regulation of neural function by influencing specific molecular and cellular pathways. Genetic expansion of a poly‐glutamine tract in human ATXN2 has been linked to several neurodegenerative diseases, where it most likely acts through gain‐of‐function effects.

 What is the molecular weight of ATXN2? 

 The molecular weight varies from 12 to 140 kDa, depending on the isoform.

 What are the isoforms of ATXN2?

 ATXN2 has many isoforms, which differ significantly in length and domain composition.

 What is the subcellular localization of ATXN2?

 According to siRNA-based experiments, it is localized in the cytoplasmic compartment of normal cells, where it mostly associates with the Golgi apparatus and stress granules (PMID: 22508507).

 What is the tissue specificity of ATXN2?

 ATXN2 protein has been detected in various tissues; however, it is most highly expressed and studied in CNS. Significant amounts of this protein can also be detected in the liver and gallbladder.

 What is the molecular function of ATXN2?

 Ataxin-2 is involved in regulating various steps of mRNA translation, including poly‐A tailing, RNA stabilization, microRNA‐dependent gene silencing, and translational activation. All those functions are linked to its interactions with the poly(A)-binding protein. Ataxin-2 is involved in the formation of stress granules and P-bodies. Furthermore, genetic models of ATXN2 loss‐of‐function have underlined the importance of ATXN2 in mTOR signaling and cellular metabolism, which are all crucial for neural homeostasis (PMID: 29869836).

 What is ATXN2's involvement in disease?

 The N-terminal region of ATXN2 normally contains a polyQ stretch of 14-31 residues that can be expanded in the pathogenic state to 32-200 residues. Intermediate length expansions of this tract increase susceptibility to amyotrophic lateral sclerosis (ALS or Lou Gehrig's disease) (PMID: 20740007). Long expansions of this tract result in spinocerebellar ataxia-2 (SCA2), an autosomal dominantly inherited, neurodegenerative disorder (PMID: 29427103). Genome-wide association studies indicate that loss-of-function mutations in this gene are associated with susceptibility to type I diabetes, obesity, and hypertension.



Protocols

Product Specific Protocols
WB protocol for Ataxin 2 antibody 21776-1-APDownload protocol
IHC protocol for Ataxin 2 antibody 21776-1-APDownload protocol
IF protocol for Ataxin 2 antibody 21776-1-APDownload protocol
IP protocol for Ataxin 2 antibody 21776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mosueWB

Mol Cell

Autism-Misregulated eIF4G Microexons Control Synaptic Translation and Higher Order Cognitive Functions.

Authors - Thomas Gonatopoulos-Pournatzis
humanWB,IF

Neuron

Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD.

Authors - Mark Y Fang
human,ratWB, CoIP

Diabetologia

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Authors - Mu Zhang
humanIF,IP

PLoS Biol

LSM12-EPAC1 defines a neuroprotective pathway that sustains the nucleocytoplasmic RAN gradient.

Authors - Jongbo Lee
mouseWB

Neurobiol Dis

Atxn2-CAG100-KnockIn mouse spinal cord shows progressive TDP43 pathology associated with cholesterol biosynthesis suppression.

Authors - Júlia Canet-Pons
  • KO Validated
mouseWB

Neurobiol Dis

Generation of an Atxn2-CAG100 knock-in mouse reveals N-acetylaspartate production deficit due to early Nat8l dysregulation.

Authors - Nesli-Ece Sen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tatyana (Verified Customer) (01-21-2023)

Very good clean signal in ICC. Labels cytoplasmic stress granules well. Antibody was incubated 2 h at RT at 1:100o dilution and could also be reused.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / SH-SY5Y cells
Ataxin 2 Antibody Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:21776-1-AP)