AURKA Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82906-1-RR (same clone as 82906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82906-1-RR (AURKA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82906-1-RR (same clone as 82906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AURKA antibody (82906-1-RR, Clone: 230087H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82906-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82906-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82906-1-RR against Human AURKA were performed. The affinity constant is below 1 pM.

AURKA Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 82906-1-PBS

Clone No.230087H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1

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Product Information

82906-1-PBS targets AURKA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0362

  Product name: Recombinant human AURKA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 108-337 aa of BC002499

  Sequence: NNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQET

• Full Name: aurora kinase A
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002499
• Gene Symbol: AURKA
• Gene ID (NCBI): 6790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14965
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).