Zinc Alpha 2 Glycoprotein Monoclonal antibody

Zinc Alpha 2 Glycoprotein Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B8A4

Cat no : 66178-1-Ig

Synonyms

AZGP1, ZA2G, ZAG, Zinc alpha 2 glycoprotein, Zinc-alpha-2-glycoprotein, Zn alpha 2 glycoprotein, Zn alpha 2 GP, ZNGP1



Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66178-1-Ig targets Zinc Alpha 2 Glycoprotein in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Zinc Alpha 2 Glycoprotein fusion protein Ag5661
Full Name alpha-2-glycoprotein 1, zinc-binding
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC033830
Gene symbol AZGP1
Gene ID (NCBI) 563
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Protocols

Product Specific Protocols
WB protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
IHC protocol for Zinc Alpha 2 Glycoprotein antibody 66178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

Reduction of AZGP1 predicts poor prognosis in esophageal squamous cell carcinoma patients in Northern China.

Authors - Hong Tang