Zinc Alpha 2 Glycoprotein Monoclonal antibody

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  WB analysis of human plasma using 66178-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66178-1-Ig (AZGP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate hyperplasia using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 66178-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 66178-1-Ig (AZGP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human breast cancer using 66178-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Zinc Alpha 2 Glycoprotein antibody (66178-1-Ig, Clone: 4B8A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Zinc Alpha 2 Glycoprotein Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66178-1-Ig

Clone No.4B8A4

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

AZGP1, Zinc-alpha-2-glycoprotein, 4B8A4, ZA2G, ZAG

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

66178-1-Ig targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5661

  Product name: Recombinant human AZGP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-298 aa of BC033830

  Sequence: MVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS

• Full Name: alpha-2-glycoprotein 1, zinc-binding
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC033830
• Gene Symbol: Zinc Alpha 2 Glycoprotein
• Gene ID (NCBI): 563
• RRID: AB_2881573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25311
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1), a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract, and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Onco Targets Ther; Reduction of AZGP1 predicts poor prognosis in esophageal squamous cell carcinoma patients in Northern China.; Authors - Hong Tang