Alpha 1 Antitrypsin Monoclonal antibody

Alpha 1 Antitrypsin Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, rat, mouse, pig







Cat no : 66135-1-Ig


A1A, A-1-Antitrypsin, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT, α1-antitrypsin

Tested Applications

Positive WB detected inhuman placenta tissue, HepG2 cells, rat liver tissue, human saliva, L02 cells, pig liver tissue, human plasma, human placenta, human milk, mouse liver tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66135-1-Ig targets Alpha 1 Antitrypsin in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha 1 Antitrypsin fusion protein Ag9516
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Calculated Molecular Weight 418 aa, 47 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC015642
Gene Symbol SERPINA1
Gene ID (NCBI) 5265
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.


Product Specific Protocols
WB protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
IHC protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
IF protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
IP protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
FC protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Liver Int

Extensively expanded murine-induced hepatic stem cells maintain high-efficient hepatic differentiation potential for repopulation of injured livers.

Authors - Bing Yu

Biochem J

LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin.

Authors - Yuan Zhang

Dis Markers

Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma

Authors - Xiubing Chen

Ann Transl Med

Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation.

Authors - Sang Luo

Ann Transl Med

Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.

Authors - Sang Luo

Blood Adv

Separate roles of LMAN1 and MCFD2 in ER-to-Golgi trafficking of FV and FVIII

Authors - Yuan Zhang