Alpha 1 Antitrypsin Monoclonal antibody
Alpha 1 Antitrypsin Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 66135-1-Ig
Validation Data Gallery
|Positive WB detected in||human placenta tissue, pig liver tissue, human saliva, rat liver tissue, HepG2 cells, L02 cells, mouse liver tissue, human milk|
|Positive IP detected in||human plasma tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66135-1-Ig is Alpha 1 Antitrypsin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat, mouse, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Alpha 1 Antitrypsin fusion protein Ag9516|
|Full Name||serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1|
|Calculated molecular weight||418 aa, 47 kDa|
|Observed molecular weight||51 kDa|
|GenBank accession number||BC015642|
|Gene ID (NCBI)||5265|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.
|Product Specific Protocols|
|WB protocol for Alpha 1 Antitrypsin antibody 66135-1-Ig||Download protocol|
|IHC protocol for Alpha 1 Antitrypsin antibody 66135-1-Ig||Download protocol|
|IF protocol for Alpha 1 Antitrypsin antibody 66135-1-Ig||Download protocol|
|IP protocol for Alpha 1 Antitrypsin antibody 66135-1-Ig||Download protocol|
|FC protocol for Alpha 1 Antitrypsin antibody 66135-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Ann Transl Med
Changes in the hepatic differentiation potential of human mesenchymal stem cells aged in vitro.
LMAN1-MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin.
Extensively expanded murine-induced hepatic stem cells maintain high-efficient hepatic differentiation potential for repopulation of injured livers.