Alpha 1 Antitrypsin Monoclonal antibody

Alpha 1 Antitrypsin Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1A9G6

Cat no : 66135-1-Ig

Synonyms

A1A, A-1-Antitrypsin, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT, α1-antitrypsin



Tested Applications

Positive WB detected inhuman placenta tissue, pig liver tissue, human saliva, rat liver tissue, HepG2 cells, L02 cells, mouse liver tissue, human milk
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 66135-1-Ig is Alpha 1 Antitrypsin Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha 1 Antitrypsin fusion protein Ag9516
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Calculated molecular weight 418 aa, 47 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC015642
Gene symbol SERPINA1
Gene ID (NCBI) 5265
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

Protocols

Product Specific Protocols
WB protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
IHC protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
IF protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
IP protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
FC protocol for Alpha 1 Antitrypsin antibody 66135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Liver Int

Extensively expanded murine-induced hepatic stem cells maintain high-efficient hepatic differentiation potential for repopulation of injured livers.

Authors - Bing Yu