Annexin A10 Monoclonal antibody

Annexin A10 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A7A11

Cat no : 66869-1-Ig

Synonyms

Annexin 10, Annexin 14, annexin A10, ANX14, ANXA10

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Validation Data Gallery

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  WB analysis of pig stomach using 66869-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66869-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66869-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 66869-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 66869-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig stomach tissue, HT-29 cells
• Positive IHC detected in: human stomach tissue, mouse pancreas tissue, human colon tissue, human colon cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66869-1-Ig targets Annexin A10 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples.

• Tested Reactivity: Human, pig, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A10 fusion protein Ag27098
• Full Name: annexin A10
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC007320
• Gene Symbol: ANXA10
• Gene ID (NCBI): 11199
• RRID: AB_2882206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA10, member of the Annexin family, is a calcium-and-phospholipid-binding protein. It is implicated in multiple physiological processes, including growth regulation, cell division, apoptosis, and differentiation. ANXA10 was recently observed to be upregulated in Barrett's esophagus, oral cancers, pancreatic cancer and colon cancer.