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WB analysis of pig stomach using 66869-1-Ig (same clone as 66869-1-PBS)
pig stomach tissue were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
WB analysis of HT-29 using 66869-1-Ig (same clone as 66869-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 66869-1-Ig (same clone as 66869-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

Annexin A10 Monoclonal antibody, PBS Only

Annexin A10 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 66869-1-PBS

Clone No.1A7A11

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Annexin 10, Annexin 14, annexin A10, ANX14, ANXA10

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66869-1-PBS targets Annexin A10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity	Human, pig, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag27098 Product name: Recombinant human ANXA10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 160-324 aa of BC007320 Sequence: QGTREEGYTDPAMAAQDAMVLWEACQQKTGEHKTMLQMILCNKSYQQLRLVFQEFQNISGQDMVDAINECYDGYFQELLVAIVLCVRDKPAYFAYRLYSAIHDFGFHNKTVIRILIARSEIDLLTIRKRYKERYGKSLFHDIRNFASGHYKKALLAICAGDAEDY Predict reactive species
Full Name	annexin A10
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC007320
Gene Symbol	ANXA10
Gene ID (NCBI)	11199
RRID	AB_2882206
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UJ72
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ANXA10, member of the Annexin family, is a calcium-and-phospholipid-binding protein. It is implicated in multiple physiological processes, including growth regulation, cell division, apoptosis, and differentiation. ANXA10 was recently observed to be upregulated in Barrett's esophagus, oral cancers, pancreatic cancer and colon cancer.