AuthentiKine™ Human beta-NGF ELISA Kit
Cat no : KE00174
Synonyms
Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF, β-NGF
Validation Data Gallery
Product Information
KE00174 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The beta-NGF ELISA kit is to be used to detect and quantify protein levels of endogenous beta -NGF. The assay recognizes human beta-NGF. An antibody specific for beta -NGF has been pre-coated onto the microwells. The beta-NGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for beta-NGF is added to detect the captured beta-NGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human beta-NGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4803 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 73%-119% |
| Cell culture supernatants | 119% | 100%-129% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 69.6 | 1.6 | 2.3 |
| 2 | 20 | 140.4 | 1.8 | 1.3 |
| 3 | 20 | 242.1 | 4.9 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 72.0 | 2.6 | 3.6 |
| 2 | 24 | 140.8 | 3.4 | 2.4 |
| 3 | 24 | 242.9 | 6.9 | 2.9 |
Background Information
NGF is a member of the neurotrophin family that regulates cell proliferation and differentiation within specific neural tissues, under both physiological and pathological condition. As NGF plays a key role in the proliferation, differentiation, and regulation of neural stem cells, several studies have indicated the clinical potential of NGF treatment in the regeneration of nerve cells following injury. NGF is initially in a 7S, 130-kDa complex of 3 proteins: alpha-NGF, beta-NGF, and gamma-NGF, while the beta subunit, exhibits all the biological activities ascribed to NGF. Beta-NGF stimulates the growth of sympathetic and some sensory neurites.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Beta nerve growth factor, Beta NGF, HSAN5, NGF, NGFB, proNGF, β-NGF |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Commun Biol Norepinephrine regulates epithelial-derived neurotrophins expression and sensory nerve regeneration through ADRB2 receptor | ||
Neuropsychiatr Dis Treat Hyperbaric Oxygen Therapy Upregulates Neurotrophic Factors to Ameliorate Post-Stroke Depression: A Randomized Sham-Controlled Trial. | ||
J Control Release M2 macrophage-derived exosomes deliver NGF-modified mRNA to promote comprehensive corneal repair. |