Tested Applications
| Positive WB detected in | A431 cells, Hepa1-6 cells, human heart tissue, human stomach tissue, HeLa cells, HepG2 cells, Jurkat cells, Raji cells, mouse spleen tissue, rat spleen tissue, L02 cells, mouse lung tissue, rat lung tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human tonsillitis tissue, human prostate cancer tissue, human oesophagus cancer tissue, human tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, NCCIT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:375-1:1500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13511-1-AP targets Beta-2-Microglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4433 Product name: Recombinant human B2M protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-119 aa of BC032589 Sequence: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM Predict reactive species |
| Full Name | beta-2-microglobulin |
| Calculated Molecular Weight | 119 aa, 14 kDa |
| Observed Molecular Weight | 12-14 kDa |
| GenBank Accession Number | BC032589 |
| Gene Symbol | B2M |
| Gene ID (NCBI) | 567 |
| ENSEMBL Gene ID | ENSG00000166710 |
| RRID | AB_2062735 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61769 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Beta-2-Microglobulin antibody 13511-1-AP | Download protocol |
| IHC protocol for Beta-2-Microglobulin antibody 13511-1-AP | Download protocol |
| IP protocol for Beta-2-Microglobulin antibody 13511-1-AP | Download protocol |
| WB protocol for Beta-2-Microglobulin antibody 13511-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-B2M monoclonal antibody (Cat# 13511-1-AP) has 33 citations across journals including Cell, Cell Research, Nature Communications, Autophagy, JCI, Science Advances, Theranostics, Clinical Cancer Research, eLife, and others. Research fields span MHC class I antigen presentation, tumor immune evasion, autophagy, cancer immunotherapy, neurodegeneration, cardiovascular repair, fibrosis, and metabolic regulation.
| Species | Application | Title |
|---|---|---|
Cell Dietary arginine drives codon-dependent MHC class I translation and improves immunity in colon tumorigenesis and respiratory viral infection. | ||
Cell IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion | ||
Cell Res Tumor PD-L1 induces β2m ubiquitylation and degradation for cancer cell immune evasion. | ||
Drug Resist Updat RNF26 regulating tumor immunogenicity of hepatocellular carcinoma by degrading GRP78 and instigating ER stress. | ||
Autophagy CircRNA GRAMD4 induces NBR1 expression to promote autophagy and immune escape in renal cell carcinoma | ||
Nat Commun Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology. |
Reviews
What customers say
The single review for 13511-1-AP is a 5-star rating from Cali Chen (October 2023). Used at a 1:2000 dilution for Western Blot on mouse brain lysate (lot 00078730), the reviewer described it as "a reliable Ab to detect endogenous B2M." Overall, the feedback is positive, highlighting its reliability for detecting endogenous levels of the target protein.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cali (Verified Customer) (10-24-2023) | A reliable Ab to detect endogenous B2M.
 |
