Validation Data Gallery
|Positive WB detected in
|A431 cells, human heart tissue, human stomach tissue, mouse lung tissue, HepG2 cells, Raji cells, mouse spleen tissue, rat spleen tissue, HeLa cells, Jurkat cells, rat lung tissue
|Positive IP detected in
|Positive IHC detected in
|human tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|A431 cells, NCCIT cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:2000-1:8000
|IF : 1:375-1:1500
|Flow Cytometry (FC)
|FC : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13511-1-AP targets Beta-2-Microglobulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Beta-2-Microglobulin fusion protein Ag4433
|Calculated molecular weight
|119 aa, 14 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
|Product Specific Protocols
|WB protocol for Beta-2-Microglobulin antibody 13511-1-AP
|IHC protocol for Beta-2-Microglobulin antibody 13511-1-AP
|IF protocol for Beta-2-Microglobulin antibody 13511-1-AP
|IP protocol for Beta-2-Microglobulin antibody 13511-1-AP
|Click here to view our Standard Protocols
Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.
Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.
Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.
Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways.
Aging (Albany NY)
β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.
B2M gene expression shapes the immune landscape of lung adenocarcinoma and determines the response to immunotherapy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Cali (Verified Customer) (10-24-2023)
A reliable Ab to detect endogenous B2M.