Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (1)

Cat no : 13511-1-AP

Synonyms

B2M, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

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Validation Data Gallery

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  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13511-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13511-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 13511-1-AP

  IP result of anti-Beta-2-Microglobulin (IP:13511-1-AP, 4ug; Detection:13511-1-AP 1:600) with A431 cells lysate 2280 ug.

• 

  IHC staining of human tonsillitis using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 13511-1-AP

  Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:750 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NCCIT using 13511-1-AP

  Immunofluorescent analysis of NCCIT cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, human heart tissue, human stomach tissue, mouse lung tissue, HepG2 cells, Raji cells, mouse spleen tissue, rat spleen tissue, HeLa cells, Jurkat cells, rat lung tissue
• Positive IP detected in: A431 cells
• Positive IHC detected in: human tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:375-1:1500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

13511-1-AP targets Beta-2-Microglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta-2-Microglobulin fusion protein Ag4433
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• Observed Molecular Weight: 12-14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• RRID: AB_2062735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Publications

Species	Application	Title
human	WB	Cell; IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion; Authors - Lina Herhaus
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
		Nat Commun; Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.; Authors - Kyota Fujita
mouse	WB	Theranostics; Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.; Authors - Jingyao Tu
	IP	Biomater Sci; Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways.; Authors - Yusuke Kambe
rat	IHC	Aging (Albany NY); β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.; Authors - Zhenchao Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cali (Verified Customer) (10-24-2023)

A reliable Ab to detect endogenous B2M.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

Beta-2-Microglobulin Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:13511-1-AP)

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