Validation Data Gallery
|Positive WB detected in||A431 cells, mouse lung tissue, HeLa cells, human heart tissue, human stomach tissue, Raji cells, HepG2 cells, mouse spleen tissue, rat spleen tissue, Jurkat cells, rat lung tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13511-1-AP targets Beta-2-Microglobulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Beta-2-Microglobulin fusion protein Ag4433|
|Calculated molecular weight||119 aa, 14 kDa|
|Observed molecular weight||12-14 kDa|
|GenBank accession number||BC032589|
|Gene ID (NCBI)||567|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
|Product Specific Protocols|
|WB protocol for Beta-2-Microglobulin antibody 13511-1-AP||Download protocol|
|IHC protocol for Beta-2-Microglobulin antibody 13511-1-AP||Download protocol|
|IF protocol for Beta-2-Microglobulin antibody 13511-1-AP||Download protocol|
|IP protocol for Beta-2-Microglobulin antibody 13511-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Aging (Albany NY)
β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.
Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9.
CNS Neurosci Ther
Impact of beta-2 microglobulin expression on the survival of glioma patients via modulating the tumor immune microenvironment.
Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair.
B2M gene expression shapes the immune landscape of lung adenocarcinoma and determines the response to immunotherapy.
Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways.