Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13511-1-AP


B2M, Beta-2-microglobulin, HDCMA22P

Tested Applications

Positive WB detected inA431 cells, mouse lung tissue, HeLa cells, human heart tissue, human stomach tissue, Raji cells, HepG2 cells, mouse spleen tissue, rat spleen tissue, Jurkat cells, rat lung tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman tonsillitis tissue, mouse lung tissue, human liver cancer tissue, human prostate cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®555 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13511-1-AP targets Beta-2-Microglobulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta-2-Microglobulin fusion protein Ag4433
Full Name beta-2-microglobulin
Calculated molecular weight 119 aa, 14 kDa
Observed molecular weight 12-14 kDa
GenBank accession numberBC032589
Gene symbol B2M
Gene ID (NCBI) 567
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.


Product Specific Protocols
WB protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
IHC protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
IF protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
IP protocol for Beta-2-Microglobulin antibody 13511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Aging (Albany NY)

β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.

Authors - Zhenchao Wu

PLoS One

Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9.

Authors - Yuanwu Ma

CNS Neurosci Ther

Impact of beta-2 microglobulin expression on the survival of glioma patients via modulating the tumor immune microenvironment.

Authors - Feng Tang

Commun Biol

Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair.

Authors - Bas Molenaar


B2M gene expression shapes the immune landscape of lung adenocarcinoma and determines the response to immunotherapy.

Authors - Yu Zhao

Biomater Sci

Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways.

Authors - Yusuke Kambe