CoraLite® Plus 647-conjugated Beta-2-Microglobulin Polyclonal antibody

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  IF Staining of A431 using CL647-13511

  Immunofluorescent analysis of (-20°C Acetone) fixed A431 cells using CoraLite® Plus 647 Beta-2-Microglobulin antibody (CL647-13511) at dilution of 1:200.

Beta-2-Microglobulin Polyclonal Antibody for IF/ICC

Cat No. CL647-13511

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

B2M, HDCMA22P, CDABP0092, Beta-2-microglobulin form pI 5.3, beta 2 microglobulin

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-13511 targets Beta-2-Microglobulin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta-2-Microglobulin fusion protein Ag4433
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• Observed Molecular Weight: 12-14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• ENSEMBL Gene ID: ENSG00000166710
• RRID: AB_2934888
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61769
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.