CoraLite® Plus 647-conjugated Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13511

Synonyms

B2M, beta 2 microglobulin, HDCMA22P

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Validation Data Gallery

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  FC experiment of HeLa using CL647-13511

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Beta-2-Microglobulin (CL647-13511) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL647-13511 targets Beta-2-Microglobulin in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta-2-Microglobulin fusion protein Ag4433
• Full Name: beta-2-microglobulin
• Calculated molecular weight: 119 aa, 14 kDa
• Observed molecular weight: 12-14 kDa
• GenBank accession number: BC032589
• Gene symbol: B2M
• Gene ID (NCBI): 567
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.