BCAT2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86367-1-RR (same clone as 86367-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86367-1-RR (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86367-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86367-1-RR against Human BCAT2 were performed. The affinity constant is below 1 pM.

BCAT2 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86367-1-PBS

Clone No.251057E6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18

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Product Information

86367-1-PBS targets BCAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9573

  Product name: Recombinant human BCAT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-392 aa of BC001900

  Sequence: MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

• Full Name: branched chain aminotransferase 2, mitochondrial
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC001900
• Gene Symbol: BCAT2
• Gene ID (NCBI): 587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15382
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).