BCKDHA Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85799-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85799-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02125-1

  Cytometric bead array standard curve of MP02125-1, BCKDHA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85799-3-PBS. Detection antibody: 85799-2-PBS. Standard: Ag32613. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP02125-2

  Cytometric bead array standard curve of MP02125-2, BCKDHA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85799-3-PBS. Detection antibody: 85799-1-PBS. Standard: Ag32613. Range: 0.625-80 ng/mL.

BCKDHA Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 85799-3-PBS

Clone No.250051G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDH E1-alpha, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC:1.2.4.4, MSU

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Product Information

85799-3-PBS targets BCKDHA as part of a matched antibody pair:

MP02125-1: 85799-3-PBS capture and 85799-2-PBS detection (validated in Cytometric bead array)

MP02125-2: 85799-3-PBS capture and 85799-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCKDHA fusion protein Ag32613
• Full Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC008933
• Gene Symbol: BCKDHA
• Gene ID (NCBI): 593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12694
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).