BCKDHA Recombinant antibody

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  WB analysis using 85799-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85799-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

BCKDHA Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85799-3-RR

Clone No.250051G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDH E1-alpha, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC:1.2.4.4, MSU

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, HEK-293T cells, HepG2 cells, rat liver tissue, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85799-3-RR targets BCKDHA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCKDHA fusion protein Ag32613
• Full Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC008933
• Gene Symbol: BCKDHA
• Gene ID (NCBI): 593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12694
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).