BCL2L15 Polyclonal antibody

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• BCL2L15 Polyclonal antibody (23975-1-AP)

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  WB analysis of SW480 using 23975-1-AP

  WB result of BCL2L15 antibody (23975-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL2L15 transfected SW480 cells.

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  IHC staining of human colon using 23975-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 23975-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23975-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 23975-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 23975-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 23975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• KD/KO Validated

BCL2L15 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 23975-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

Bcl-2 family kin, Bcl2-L-15, Bcl-2-like protein 15, Bfk, C1orf178

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Tested Applications

• Positive IHC detected in: human colon tissue, human appendicitis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

23975-1-AP targets BCL2L15 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21155

  Product name: Recombinant human BCL2L15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-163 aa of BC127719

  Sequence: MKSSQTFEEQTECIVNTLLMDFLSPTLQVASRNLCCVDEVDSGEPCSFDVAIIAGRLRMLGDQFNGELEASAKNVIAETIKGQTGAILQNTVESLSKTWCAQDSSLAYERAFLAVSVKLLEYMAHIAPEVVGQVAIPMTGMINGNQAIREFIQGQGGWENLES

• Full Name: BCL2-like 15
• Calculated Molecular Weight: 163 aa, 18 kDa
• GenBank Accession Number: BC127719
• Gene Symbol: BCL2L15
• Gene ID (NCBI): 440603
• RRID: AB_3085718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q5TBC7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B-cell lymphoma 2 (Bcl-2)-like protein 15 (BCL2L15), also known as BCL2 family kin (BFK), belongs to the BCL2 protein family, and appears to induce weak apoptosis and antagonize the pro-survival function of Bcl2 (PMID:32708974). In addition, studies reported BCL2L15 expression in the stomach, ovaries, bone marrow, spleen, and uterus (PMID:12700646).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	J Transl Med; CTPAD: an interactive web application for comprehensive transcriptomic profiling in allergic diseases; Authors - Suizi Zhou