BCL3 Recombinant antibody

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  IHC staining of mouse spleen using 84882-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84882-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84882-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84882-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84882-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84882-5-RR against Human BCL3 were performed. The affinity constant is 0.581 nM.

BCL3 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84882-5-RR

Clone No.242233B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

242233B11, B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37

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Tested Applications

• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84882-5-RR targets BCL3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCL3 fusion protein Ag13375
• Full Name: B-cell CLL/lymphoma 3
• Calculated Molecular Weight: 446 aa, 47 kDa
• Observed Molecular Weight: 48-60 kDa
• GenBank Accession Number: BC064993
• Gene Symbol: BCL3
• Gene ID (NCBI): 602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P20749
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain (PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248).