BCL6 Polyclonal antibody

• Featured Product
• KD/KO Validated

BCL6 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 21187-1-AP

Synonyms

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51

---

Validation Data Gallery

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 21187-1-AP

  IP Result of anti-BCL6 (IP:21187-1-AP, 4ug; Detection:21187-1-AP 1:1000) with Raji cells lysate 1400ug.

• 

  IHC staining of human lymphoma using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  FC experiment of Ramos using 21187-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.2 ug Anti-Human BCL6 (21187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive WB detected in: Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below

Product Information

The immunogen of 21187-1-AP is BCL6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL6 fusion protein Ag15519
• Full Name: B-cell CLL/lymphoma 6
• Calculated molecular weight: 706 aa, 79 kDa
• Observed molecular weight: 79-95 kDa
• GenBank accession number: BC150184
• Gene symbol: BCL6
• Gene ID (NCBI): 604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

Publications

Species	Application	Title
	IHC	Nat Commun; A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.; Authors - Christine Quach
mouse	WB	Sci Rep; Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.; Authors - Yanhong Shi
human	IHC	Front Cell Dev Biol; The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.; Authors - Zhengyan Chang
human	IHC	Front Oncol; Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.; Authors - Shuang Wen
mouse	WB	Int Immunopharmacol; Sunitinib malate inhibits intestinal tumor development in male ApcMin/+ mice by down-regulating inflammation-related factors with suppressing β-cateinin/c-Myc pathway and re-balancing Bcl-6 and Caspase-3.; Authors - Lai Chen
human	WB	Placenta; MiR-187 regulates the proliferation, migration and invasion of human trophoblast cells by repressing BCL6-mediated activation of PI3K/AKT signaling.; Authors - Xin Chen

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011