Validation Data Gallery
|Positive WB detected in||Raji cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human lymphoma tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Ramos cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21187-1-AP is BCL6 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCL6 fusion protein Ag15519|
|Full Name||B-cell CLL/lymphoma 6|
|Calculated molecular weight||706 aa, 79 kDa|
|Observed molecular weight||79-95 kDa|
|GenBank accession number||BC150184|
|Gene ID (NCBI)||604|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.
|Product Specific Protocols|
|WB protocol for BCL6 antibody 21187-1-AP||Download protocol|
|IHC protocol for BCL6 antibody 21187-1-AP||Download protocol|
|IP protocol for BCL6 antibody 21187-1-AP||Download protocol|
|FC protocol for BCL6 antibody 21187-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Methods Mol Biol
Conditional Knockout Mouse Models to Study the Roles of Individual NF-κB Transcription Factors in Lymphocytes.
Cell Death Dis
B-cell lymphoma 6 alleviates nonalcoholic fatty liver disease in mice through suppression of fatty acid transporter CD36.
Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis.
Japanese encephalitis virus induces apoptosis by inhibiting Foxo signaling pathway.
Eur J Pharmacol
Steroid receptor coactivator-3 is a pivotal target of gambogic acid in B-cell Non-Hodgkin lymphoma and an inducer of histone H3 deacetylation.
Biochem Biophys Res Commun
CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p.