BCL6 Polyclonal antibody

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  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21187-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21187-1-AP (BCL6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 21187-1-AP

  IP result of anti-BCL6 (IP:21187-1-AP, 4ug; Detection:21187-1-AP 1:1000) with Raji cells lysate 1400ug.

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  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21187-1-AP (BCL6 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 21187-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21187-1-AP (BCL6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• Featured Product
• KD/KO Validated

BCL6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21187-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

BCL-6, BCL-5, BCL 6, B-cell lymphoma 6 protein, B-cell lymphoma 5 protein

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:3000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

21187-1-AP targets BCL6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL6 fusion protein Ag15519
• Full Name: B-cell CLL/lymphoma 6
• Calculated Molecular Weight: 706 aa, 79 kDa
• Observed Molecular Weight: 79-95 kDa
• GenBank Accession Number: BC150184
• Gene Symbol: BCL6
• Gene ID (NCBI): 604
• RRID: AB_2878827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41182
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

Publications

Species	Application	Title
	IHC	Nat Commun; A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.; Authors - Christine Quach
human	WB	Respir Res; Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis.; Authors - Ziyan Yan
mouse	WB	Sci Rep; Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.; Authors - Yanhong Shi
human	WB,IP	Front Cell Dev Biol; Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma; Authors - Mayuko Nishi
human	IHC	Front Cell Dev Biol; The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.; Authors - Zhengyan Chang
human	IHC	Front Oncol; Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.; Authors - Shuang Wen