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BCL6 Polyclonal antibody
BCL6 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (1)
WB, IP, IHC, FC, ELISA
Cat no : 21187-1-AP
Validation Data Gallery
|Positive WB detected in||Raji cells|
|Positive IP detected in||Raji cells|
|Positive IHC detected in||human tonsillitis tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Ramos cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 9 publications below|
|IHC||See 4 publications below|
The immunogen of 21187-1-AP is BCL6 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCL6 fusion protein Ag15519|
|Full Name||B-cell CLL/lymphoma 6|
|Calculated molecular weight||706 aa, 79 kDa|
|Observed molecular weight||79-95 kDa|
|GenBank accession number||BC150184|
|Gene ID (NCBI)||604|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.
|Product Specific Protocols|
|WB protocol for BCL6 antibody 21187-1-AP||Download protocol|
|IHC protocol for BCL6 antibody 21187-1-AP||Download protocol|
|IP protocol for BCL6 antibody 21187-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.
Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.
Front Cell Dev Biol
The Construction and Analysis of ceRNA Network and Patterns of Immune Infiltration in Colon Adenocarcinoma Metastasis.
Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney.
Sunitinib malate inhibits intestinal tumor development in male ApcMin/+ mice by down-regulating inflammation-related factors with suppressing β-cateinin/c-Myc pathway and re-balancing Bcl-6 and Caspase-3.
MiR-187 regulates the proliferation, migration and invasion of human trophoblast cells by repressing BCL6-mediated activation of PI3K/AKT signaling.