BCL6 Polyclonal antibody

BCL6 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 21187-1-AP

Synonyms

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51



Tested Applications

Positive WB detected inRaji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21187-1-AP is BCL6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL6 fusion protein Ag15519
Full Name B-cell CLL/lymphoma 6
Calculated molecular weight 706 aa, 79 kDa
Observed molecular weight 79-95 kDa
GenBank accession numberBC150184
Gene symbol BCL6
Gene ID (NCBI) 604
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCl6, a zinc finger transcription factor, contains a N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

Protocols

Product Specific Protocols
WB protocol for BCL6 antibody 21187-1-APDownload protocol
IHC protocol for BCL6 antibody 21187-1-APDownload protocol
IP protocol for BCL6 antibody 21187-1-APDownload protocol
FC protocol for BCL6 antibody 21187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Methods Mol Biol

Conditional Knockout Mouse Models to Study the Roles of Individual NF-κB Transcription Factors in Lymphocytes.

Authors - Emma J Adams
human,mouseWB

Cell Death Dis

B-cell lymphoma 6 alleviates nonalcoholic fatty liver disease in mice through suppression of fatty acid transporter CD36.

Authors - Hao Zhang
  • KD Validated
humanWB

Respir Res

Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial-mesenchymal transition during radiation-induced pulmonary fibrosis.

Authors - Ziyan Yan
humanWB

Vet Microbiol

Japanese encephalitis virus induces apoptosis by inhibiting Foxo signaling pathway.

Authors - Fenglin Guo
humanWB

Eur J Pharmacol

Steroid receptor coactivator-3 is a pivotal target of gambogic acid in B-cell Non-Hodgkin lymphoma and an inducer of histone H3 deacetylation.

Authors - Zichu Zhao
humanWB

Biochem Biophys Res Commun

CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p.

Authors - Ranran Sun