BCR Polyclonal antibody

• 

  WB analysis of Y79 using 22585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22585-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 22585-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22585-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22585-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 22585-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22585-1-AP

  IP result of anti-BCR (IP:22585-1-AP, 4ug; Detection:22585-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

• 

  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 22585-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 22585-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BCR antibody (22585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HepG2 using 22585-1-AP

  Immunofluorescent analysis of HepG2 cells, using BCR antibody 22585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

BCR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22585-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Renal carcinoma antigen NY-REN-26, EC:2.7.11.1, breakpoint cluster region, BCR1, BCR 1

---

Tested Applications

• Positive WB detected in: Y79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

22585-1-AP targets BCR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18281

  Product name: Recombinant human BCR protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC066122

  Sequence: MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQSIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQS

• Full Name: breakpoint cluster region
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC066122
• Gene Symbol: BCR
• Gene ID (NCBI): 613
• RRID: AB_2879127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P11274
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IHC	Neurogastroenterol Motil; Loss function of Bcr mutation causes gastrointestinal dysmotility and brain developmental defects.; Authors - Yongtao Xiao ; KO Validated
mouse	WB	Nat Commun; Capture of RNA-binding proteins across mouse tissues using HARD-AP; Authors - Yijia Ren

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Costanza (Verified Customer) (09-27-2022)

Good Immunofluorescence from paraffin sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100