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  • KD/KO Validated

BCRP,ABCG2 Polyclonal antibody

BCRP,ABCG2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27286-1-AP

Synonyms

ABC15, ABCG2, ABCP, BCRP,ABCG2, BMDP, CD338, CDw338, EST157481, MRX, MXR, MXR1



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27286-1-AP is BCRP,ABCG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCRP,ABCG2 fusion protein Ag25911
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Calculated molecular weight 70 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC021281
Gene symbol ABCG2
Gene ID (NCBI) 9429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

Protocols

Product Specific Protocols
WB protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
IHC protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Mol Cancer

Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.

Authors - Jianyou Gu
human,mouseWB,IHC

Mol Ther

CRISPR-mediated MECOM depletion retards tumor growth by reducing cancer stem cell properties in lung squamous cell carcinoma.

Authors - Yuanyuan Ma
humanWB

Environ Pollut

Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.

Authors - Jianqiang Zhu
humanIHC

Pharmacol Res

PMID: 31408695

Authors - Jianli Guo
mouseWB

J Ethnopharmacol

Fufang Zhenzhu Tiaozhi capsule ameliorates hyperuricemic nephropathy by inhibition of PI3K/AKT/NF-κB pathway

Authors - Ming-Hui Li
humanWB

Food Funct

S-20, a steroidal saponin from the berries of black nightshade, exerts anti-multidrug resistance activity in K562/ADR cells through autophagic cell death and ERK activation.

Authors - Yi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (09-12-2022)

Used in Western Blot at 1:3000 Dilution and it detected a band at 75 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: U2OS Osteosarcoma cells
BCRP,ABCG2 Antibody Western Blot validation (1:3000 dilution) in U2OS Osteosarcoma cells (Cat no:27286-1-AP)