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  • KD/KO Validated

BCRP,ABCG2 Polyclonal antibody

BCRP,ABCG2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 27286-1-AP


ABC15, ABCG2, ABCP, BCRP,ABCG2, BMDP, CD338, CDw338, EST157481, MRX, MXR, MXR1

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27286-1-AP is BCRP,ABCG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCRP,ABCG2 fusion protein Ag25911
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Calculated molecular weight 70 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC021281
Gene symbol ABCG2
Gene ID (NCBI) 9429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.


Product Specific Protocols
WB protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
IHC protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Udesh (Verified Customer) (09-12-2022)

Used in Western Blot at 1:3000 Dilution and it detected a band at 75 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: U2OS Osteosarcoma cells
BCRP,ABCG2 Antibody Western Blot validation (1:3000 dilution) in U2OS Osteosarcoma cells (Cat no:27286-1-AP)