|Positive WB detected in||HepG2 cells, HEK-293 cells, MCF-7 cells|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 27286-1-AP is BCRP,ABCG2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCRP,ABCG2 fusion protein Ag25911|
|Full Name||ATP-binding cassette, sub-family G (WHITE), member 2|
|Calculated molecular weight||70 kDa|
|Observed molecular weight||68-72 kDa|
|GenBank accession number||BC021281|
|Gene ID (NCBI)||9429|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
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