Validation Data Gallery
|Positive WB detected in||HepG2 cells, HEK-293 cells, MCF-7 cells, SGC-7901 cells, mouse brain tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
27286-1-AP targets BCRP,ABCG2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCRP,ABCG2 fusion protein Ag25911|
|Full Name||ATP-binding cassette, sub-family G (WHITE), member 2|
|Calculated molecular weight||70 kDa|
|Observed molecular weight||68-72 kDa|
|GenBank accession number||BC021281|
|Gene ID (NCBI)||9429|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.
Drug Resist Updat
Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer
Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.
A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance
CRISPR-mediated MECOM depletion retards tumor growth by reducing cancer stem cell properties in lung squamous cell carcinoma.
Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice
Pleckstrin homology and RhoGEF domain containing G4 (PLEKHG4) leads to the activation of RhoGTPases promoting the malignant phenotypes of thyroid cancer
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (09-12-2022)
Used in Western Blot at 1:3000 Dilution and it detected a band at 75 kDa.