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  • KD/KO Validated

BCRP,ABCG2 Polyclonal antibody

BCRP,ABCG2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27286-1-AP

Synonyms

ABC15, ABCG2, ABCP, BCRP,ABCG2, BMDP, CD338, CDw338, EST157481, MRX, MXR, MXR1



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27286-1-AP is BCRP,ABCG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCRP,ABCG2 fusion protein Ag25911
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Calculated molecular weight 70 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC021281
Gene symbol ABCG2
Gene ID (NCBI) 9429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

Protocols

Product Specific Protocols
WB protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
IHC protocol for BCRP,ABCG2 antibody 27286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Eur J Pharmacol

Dual actions of norathyriol as a new candidate hypouricaemic agent: uricosuric effects and xanthine oxidase inhibition.

Authors - Hua Lin
humanWB

Cell Death Dis

UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop.

Authors - Xu Liu
humanWB

Cancers (Basel)

Preclinical Evaluation of the Novel Small-Molecule MSI-N1014 for Treating Drug-Resistant Colon Cancer via the LGR5/β-catenin/miR-142-3p Network and Reducing Cancer-Associated Fibroblast Transformation.

Authors - Vijesh Kumar Yadav
humanWB

Cell Death Dis

1,25-dihydroxyvitamin D3 signaling-induced decreases in IRX4 inhibits NANOG-mediated cancer stem-like properties and gefitinib resistance in NSCLC cells.

Authors - Zhirong Jia
humanWB

Environ Toxicol

Ginsenoside Rg3 attenuates the osimertinib resistance by reducing the stemness of non-small cell lung cancer cells.

Authors - Qinquan Tan
mouseWB

Eur J Pharmacol

Mangiferin promotes intestinal elimination of uric acid by modulating intestinal transporters.

Authors - Qiurui Li