BCRP,ABCG2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BCRP,ABCG2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 27286-1-AP

Synonyms

ABC15, ABCG2, ABCP, BCRP,ABCG2, BMDP, CD338, CDw338, EST157481, MRX, MXR, MXR1

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Validation Data Gallery

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  WB analysis using 27286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27286-1-AP

  WB result of BCRP,ABCG2 antibody (27286-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCRP,ABCG2 transfected MCF-7 cells.

• 

  IHC staining of human liver cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27286-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 25 publications below
• IHC: See 5 publications below

Product Information

The immunogen of 27286-1-AP is BCRP,ABCG2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCRP,ABCG2 fusion protein Ag25911
• Full Name: ATP-binding cassette, sub-family G (WHITE), member 2
• Calculated molecular weight: 70 kDa
• Observed molecular weight: 68-72 kDa
• GenBank accession number: BC021281
• Gene symbol: ABCG2
• Gene ID (NCBI): 9429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

Publications

Species	Application	Title
human,mouse	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human,mouse	WB,IHC	Mol Ther; CRISPR-mediated MECOM depletion retards tumor growth by reducing cancer stem cell properties in lung squamous cell carcinoma.; Authors - Yuanyuan Ma
human	WB	Environ Pollut; Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.; Authors - Jianqiang Zhu
human	IHC	Pharmacol Res; PMID: 31408695; Authors - Jianli Guo
mouse	WB	J Ethnopharmacol; Fufang Zhenzhu Tiaozhi capsule ameliorates hyperuricemic nephropathy by inhibition of PI3K/AKT/NF-κB pathway; Authors - Ming-Hui Li
human	WB	Food Funct; S-20, a steroidal saponin from the berries of black nightshade, exerts anti-multidrug resistance activity in K562/ADR cells through autophagic cell death and ERK activation.; Authors - Yi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (09-12-2022)

Used in Western Blot at 1:3000 Dilution and it detected a band at 75 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS Osteosarcoma cells

BCRP,ABCG2 Antibody Western Blot validation (1:3000 dilution) in U2OS Osteosarcoma cells (Cat no:27286-1-AP)